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. 1972 Oct;10(4):668–674. doi: 10.1128/jvi.10.4.668-674.1972

Behavior of Coliphage Lambda in Shigella flexneri 2a

P Gemski Jr 1, J A Alexeichik 1, L S Baron 1
PMCID: PMC356519  PMID: 4563593

Abstract

The insensitivity of wild-type Shigella flexneri 2a to coliphage λ is a consequence of its native genetic defect in the malA gene cluster. The “smooth” S. flexneri 2a lipopolysaccharide layer affects the efficient adsorption of λ. Derivatives, capable of serving as functional hosts for λ, were obtained by repairing the malA lesion, enabling the expression of the malB-λrcp region of S. flexneri. Introduction of a mutation into S. flexneri causing a “rough” lipopolysaccharide character resulted in more efficient adsorption of λ. Such S. flexneri hosts can be stably lysogenized and upon induction yield gal+-transducing lysates. Lambda propagated on a malA+ rough S. flexneri host was restricted by Escherichia coli K-12 and E. coli B, but not by E. coli C. This S. flexneri host did not restrict λ grown on these E. coli strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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