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Journal of Virology logoLink to Journal of Virology
. 1972 Oct;10(4):675–682. doi: 10.1128/jvi.10.4.675-682.1972

Transcription of the Herpes Simplex Virus Genome in Human Cells

Edward K Wagner 1, Ronald I Swanstrom 1, Marvin G Stafford 1
PMCID: PMC356520  PMID: 4343545

Abstract

We have examined the details of the transcription of the herpes simplex virus (HSV) genome in HeLa cells using deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization. The following findings are reported. (i) Virus-specific RNA (vRNA) synthesized following onset of HSV-DNA replication (L-vRNA) is complementary to as much as 90% of the HSV genome. (ii) There is no significant class of virus-specific RNA synthesized later than L-vRNA. (iii) The vRNA synthesized prior to HSV-DNA replication (E-vRNA) is composed of two classes; one class comprising 75% of the total E-vRNA is found in large amounts as early as 45 min after infection, whereas the other class making up the other 25% of E-vRNA is found in only small amounts at 1.5 hr after infection. This second class of E-vRNA is found in amounts comparable to the first by 3.5 hr after infection. (iv) Inhibition of HSV-DNA synthesis results in the continued synthesis of E-vRNA, but there is no synthesis of L-vRNA. (v) Finally, there is no class of vRNA found in the nucleus that is not found associated with cytoplasmic polyribosomes either early or late after infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borun T. W., Scharff M. D., Robbins E. Preparation of mammalian polyribosomes with the detergent Nonidet P-40. Biochim Biophys Acta. 1967 Nov 21;149(1):302–304. doi: 10.1016/0005-2787(67)90715-0. [DOI] [PubMed] [Google Scholar]
  2. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  3. Kates J. R., McAuslan B. R. Poxvirus DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1967 Jul;58(1):134–141. doi: 10.1073/pnas.58.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lucas J. J., Ginsberg H. S. Synthesis of virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus. J Virol. 1971 Aug;8(2):203–214. doi: 10.1128/jvi.8.2.203-214.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pfeiffer S. E., Tolmach L. J. Inhibition of DNA synthesis in HeLa cells by hydroxyurea. Cancer Res. 1967 Jan;27(1):124–129. [PubMed] [Google Scholar]
  7. ROIZMAN B. Polykaryocytosis induced by viruses. Proc Natl Acad Sci U S A. 1962 Feb;48:228–234. doi: 10.1073/pnas.48.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sauer G., Kidwai J. R. The transcription of the SV40 genome in productively infected and transformed cells. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1256–1263. doi: 10.1073/pnas.61.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thomas D. C., Green M. Biochemical studies on adenovirus multiplication. XV. Transcription of the adenovirus type II genome during productive infection. Virology. 1969 Oct;39(2):205–210. doi: 10.1016/0042-6822(69)90040-3. [DOI] [PubMed] [Google Scholar]
  10. Wagner E. K. Evidence for transcriptional control of the herpes simplex virus genome in infected human cells. Virology. 1972 Feb;47(2):502–506. doi: 10.1016/0042-6822(72)90289-9. [DOI] [PubMed] [Google Scholar]
  11. Wagner E. K., Roizman B. Effect of the Vinca alkaloids on RNA synthesis in human cells in vitro. Science. 1968 Nov 1;162(3853):569–570. doi: 10.1126/science.162.3853.569. [DOI] [PubMed] [Google Scholar]
  12. Wagner E. K., Roizman B. RNA synthesis in cells infected with herpes simplex virus. II. Evidence that a class of viral mRNA is derived from a high molecular weight precursor synthesized in the nucleus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):626–633. doi: 10.1073/pnas.64.2.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wagner E. K., Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus. I. Patterns of ribonucleic acid synthesis in productively infected cells. J Virol. 1969 Jul;4(1):36–46. doi: 10.1128/jvi.4.1.36-46.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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