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. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972

Structure of the Bluetongue Virus Capsid

D W Verwoerd 1, H J Els 1, Ethel-Michéle De Villiers 1, H Huismans 1
PMCID: PMC356534  PMID: 4117349

Abstract

Seven polypeptides were found to be present in the capsid of the bluetongue virus (BTV), four of which are major and three are minor components. This number and size distribution is the same as that found in reovirus, which has a similar segmented, double-stranded ribonucleic acid genome. In both viruses an excellent correlation is found between the molecular weights of certain genome segments and those of the polypeptides, suggesting a direct coding relationship between them. Careful comparison, however, indicates that the correlation patterns of the two viruses are different, suggesting that different genome segments may be utilized to code for certain capsid proteins. This phenomenon may be related to the fact that these viruses can be distinguished morphologically, reovirus having a larger diameter and possessing a double-layered capsid against the smaller single capsid of the BTV. Utilizing the degrading effect of CsCl gradients, it was demonstrated, however, that two of the BTV polypeptides are present as a diffuse protein layer surrounding the capsid. This outer layer probably has some of the functions of the reovirus outer capsid, one being the “masking” of a viral transcriptase which could be demonstrated after its removal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amano Y., Katagiri S., Ishida N., Watanabe Y. Spontaneous degradation of reovirus capsid into subunits. J Virol. 1971 Nov;8(5):805–808. doi: 10.1128/jvi.8.5.805-808.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borsa J., Graham A. F. Reovirus: RNA polymerase activity in purified virions. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895–901. doi: 10.1016/0006-291x(68)90396-3. [DOI] [PubMed] [Google Scholar]
  3. Bowne J. G., Ritchie A. E. Some morphological features of bluetongue virus. Virology. 1970 Apr;40(4):903–911. doi: 10.1016/0042-6822(70)90136-4. [DOI] [PubMed] [Google Scholar]
  4. Chang C. T., Zweerink H. J. Fate of parental reovirus in infected cell. Virology. 1971 Dec;46(3):544–555. doi: 10.1016/0042-6822(71)90058-4. [DOI] [PubMed] [Google Scholar]
  5. Cordell-Stewart B., Taylor M. W. Effect of double-stranded viral RNA on mammalian cells in culture. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1326–1330. doi: 10.1073/pnas.68.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dunker A. K., Rueckert R. R. Observations on molecular weight determinations on polyacrylamide gel. J Biol Chem. 1969 Sep 25;244(18):5074–5080. [PubMed] [Google Scholar]
  7. Els H. J., Verwoerd D. W. Morphology of bluetongue virus. Virology. 1969 Jun;38(2):213–219. doi: 10.1016/0042-6822(69)90362-6. [DOI] [PubMed] [Google Scholar]
  8. Howell P. G., Verwoerd D. W., Oellermann R. A. Plaque formation by bluetongue virus. Onderstepoort J Vet Res. 1967 Dec;34(2):317–332. [PubMed] [Google Scholar]
  9. Huismans H. Host cell protein synthesis after infection with bluetongue virus and reovirus. Virology. 1971 Nov;46(2):500–503. doi: 10.1016/0042-6822(71)90053-5. [DOI] [PubMed] [Google Scholar]
  10. Kapuler A. M. An extraordinary temperature dependence of the reovirus transcriptase. Biochemistry. 1970 Oct 27;9(22):4453–4457. doi: 10.1021/bi00824a029. [DOI] [PubMed] [Google Scholar]
  11. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loh P. C., Shatkin A. J. Structural proteins of reoviruses. J Virol. 1968 Nov;2(11):1353–1359. doi: 10.1128/jvi.2.11.1353-1359.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy F. A., Borden E. C., Shope R. E., Harrison A. Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. Electron microscopic studies. J Gen Virol. 1971 Nov;13(2):273–288. doi: 10.1099/0022-1317-13-2-273. [DOI] [PubMed] [Google Scholar]
  14. Shatkin A. J., Sipe J. D., Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Skehel J. J., Joklik W. K. Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores. Virology. 1969 Dec;39(4):822–831. doi: 10.1016/0042-6822(69)90019-1. [DOI] [PubMed] [Google Scholar]
  17. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  18. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. VASQUEZ C., TOURNIER P. NEW INTERPRETATION OF THE REOVIRUS STRUCTURE. Virology. 1964 Sep;24:128–130. doi: 10.1016/0042-6822(64)90162-x. [DOI] [PubMed] [Google Scholar]
  20. Verwoerd D. W. Failure to demonstrate in vitro as opposed to in vivo transcription of the bluetongue virus genome. Onderstepoort J Vet Res. 1970 Dec;37(4):225–227. [PubMed] [Google Scholar]
  21. Verwoerd D. W., Louw H., Oellermann R. A. Characterization of bluetongue virus ribonucleic acid. J Virol. 1970 Jan;5(1):1–7. doi: 10.1128/jvi.5.1.1-7.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Verwoerd D. W. Purification and characterization of bluetongue virus. Virology. 1969 Jun;38(2):203–212. doi: 10.1016/0042-6822(69)90361-4. [DOI] [PubMed] [Google Scholar]
  23. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  24. Zweerink H. J., Joklik W. K. Studies on the intracellular synthesis of reovirus-specified proteins. Virology. 1970 Jul;41(3):501–518. doi: 10.1016/0042-6822(70)90171-6. [DOI] [PubMed] [Google Scholar]
  25. Zweerink H. J., McDowell M. J., Joklik W. K. Essential and nonessential noncapsid reovirus proteins. Virology. 1971 Sep;45(3):716–723. doi: 10.1016/0042-6822(71)90185-1. [DOI] [PubMed] [Google Scholar]

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