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. 1972 Oct;10(4):824–834. doi: 10.1128/jvi.10.4.824-834.1972

Electron Microscopical and Biochemical Characterization of Infectious Pancreatic Necrosis Virus

Russell K Kelly 1, Philip C Loh 1
PMCID: PMC356539  PMID: 4117351

Abstract

An electron microscopical and biochemical examination of the properties of infectious pancreatic necrosis virus (IPN) and of its ribonucleic acid (RNA) was made. The buoyant density of IPN in CsCl was found to be 1.33 g/cm3. Electron microscopical examination of the banded virus revealed structures similar in size (74 nm) and shape to reoviruses but lacking a characteristic inner capsid structure. Polyacrylamide gel electrophoretic analysis of IPN-RNA revealed a single non-segmented component of molecular weight 3.2 × 106. Its susceptibility to ribonuclease, base composition, and resistance to thermal denaturation indicated a single-stranded RNA structure. However, its sedimentation behavior (16S) independent of ionic strength in sucrose gradients, partial solubility in 2 m LiCl, and ribonuclease resistance in the presence of Mg2+ suggest an unusual secondary structure of unknown nature. The accumulated data indicate that IPN virus does not belong to either the picornavirus or reovirus groups and may represent a new group of viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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