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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Science. 2012 Jun 29;336(6089):1726–1729. doi: 10.1126/science.1216848

Fig. 3.

Fig. 3

Unwinding characteristics for different sequences and conditions. (A) Distribution of the number of steps for the standard substrate (60% GC content) (light gray: at 22 °C; dark gray: 9 °C, both in 100 µM MgCl2). (B) Distribution of the number of steps for the AU-rich construct (light gray: 23°C; dark gray: 9°C, both in 5 mM MgCl2). (C–D) Representative four and five - step patterns, respectively, obtained from AU-rich construct in 5 mM MgCl2 at 9 °C. (E) Unwinding for different constructs and temperatures. (F) Unwinding time vs. NaCl concentration for the standard substrate.