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. 1972 Oct;10(4):885–888. doi: 10.1128/jvi.10.4.885-888.1972

Reversible Inactivation of the Deoxyribonucleic Acid Polymerase of Rauscher Leukemia Virus

Steven R Tronick 1, Edward M Scolnick 1, Wade P Parks 1
PMCID: PMC356550  PMID: 4117352

Abstract

Rauscher leukemia virus deoxyribonucleic acid polymerase is reversibly inactivated by 6 m guanidine-hydrochloride. Gel filtration in 6 m guanidine-hydrochloride reveals that the viral deoxyribonucleic acid polymerase consists of a single polypeptide chain of approximately 70,000 molecular weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Duesberg P., Helm K. V., Canaani E. Properties of a soluble DNA polymerase isolated from Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):747–751. doi: 10.1073/pnas.68.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fish W. W., Mann K. G., Tanford C. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem. 1969 Sep 25;244(18):4989–4994. [PubMed] [Google Scholar]
  3. Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hurwitz J., Leis J. P. RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction. J Virol. 1972 Jan;9(1):116–129. doi: 10.1128/jvi.9.1.116-129.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kacian D. L., Watson K. F., Burny A., Spiegelman S. Purification of the DNA polymerase of avian myeloblastosis virus. Biochim Biophys Acta. 1971 Sep 24;246(3):365–383. doi: 10.1016/0005-2787(71)90773-8. [DOI] [PubMed] [Google Scholar]
  6. Klotz I. M., Darnall D. W. Protein subunits: a table (second edition). Science. 1969 Oct 3;166(3901):126–128. doi: 10.1126/science.166.3901.126. [DOI] [PubMed] [Google Scholar]
  7. Leis J. P., Hurwitz J. RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction. J Virol. 1972 Jan;9(1):130–142. doi: 10.1128/jvi.9.1.130-142.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parks W. P., Scolnick E. M., Ross J., Todaro G. J., Aaronson S. A. Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses. J Virol. 1972 Jan;9(1):110–115. doi: 10.1128/jvi.9.1.110-115.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ross J., Scolnick E. M., Todaro G. J., Aaronson S. A. Separation of murine cellular and murine leukaemia virus DNA polymerases. Nat New Biol. 1971 Jun 9;231(23):163–167. doi: 10.1038/newbio231163a0. [DOI] [PubMed] [Google Scholar]
  10. Scolnick E. M., Parks W. P., Todaro G. J., Aaronson S. A. Immunological characterization of primate C-type virus reverse transcriptases. Nat New Biol. 1972 Jan 12;235(54):35–40. doi: 10.1038/newbio235035a0. [DOI] [PubMed] [Google Scholar]
  11. Tanford C. Protein denaturation. Adv Protein Chem. 1968;23:121–282. doi: 10.1016/s0065-3233(08)60401-5. [DOI] [PubMed] [Google Scholar]

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