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. 1972 Nov;10(5):896–901. doi: 10.1128/jvi.10.5.896-901.1972

Cellular Alteration by Interferon: a Virus-Free System for Assaying Interferon

William E Stewart II 1, Erik De Clercq 1, Pierre De Somer 1
PMCID: PMC356556  PMID: 4344256

Abstract

A system is described for assaying mouse interferon without using a viral “challenge” agent. Interferon-treated L cells were destroyed by exposure to polyriboinosinic·polyribocytidylic acid [poly(I)·poly(C)], and the amount of destruction was dependent on both the concentration of interferon to which the cells were exposed and the amount of poly(I)·poly(C) used as the “challenge” material. If the amount of poly(I)·poly(C) was constant, the concentration of interferon could be determined by quantitating cell destruction 6 hr after addition of the double-stranded ribonucleic acid. In addition to eliminating the necessity for employing infectious virus for interferon assays, this system has the advantages of being quicker, easier, and more sensitive than other interferon assays. The sensitivity of the assay is related directly to the amount of poly(I)·poly(C) applied to the cells, with each fivefold increase of poly(I)·poly(C) giving about a fivefold increase of sensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen P. T., Giron D. J. Rapid sensitive assay for interferons based on the inhibition of MM virus nucleic acid synthesis. Appl Microbiol. 1970 Sep;20(3):317–322. doi: 10.1128/am.20.3.317-322.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FINTER N. B. QUANTITATIVE HAEMADSORPTION, A NEW ASSAY TECHNIQUE. I. ASSAY OF INTERFERON. Virology. 1964 Dec;24:589–597. doi: 10.1016/0042-6822(64)90212-0. [DOI] [PubMed] [Google Scholar]
  4. GIFFORD G. E., MUSSETT M. V., HELLER E. COMPARATIVE STUDIES ON THE PRODUCTION AND ASSAY OF INTERFERON. J Gen Microbiol. 1964 Mar;34:475–481. doi: 10.1099/00221287-34-3-475. [DOI] [PubMed] [Google Scholar]
  5. Gresser I., Brouty-Boyé D., Thomas M. T., Macieira-Coelho A. Interferon and cell division. I. Inhibition of the multiplication of mouse leukemia L 1210 cells in vitro by interferon preparations. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1052–1058. doi: 10.1073/pnas.66.4.1052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gresser I., Brouty-Boyé D., Thomas M. T., Macieira-Coelho A. Interferon and cell division. II. Influence of various experimental conditions on the inhibition of L1210 cell multiplication in vitro by interferon preparations. J Natl Cancer Inst. 1970 Dec;45(6):1145–1153. [PubMed] [Google Scholar]
  7. Gresser I., Thomas M. T., Brouty-Boyé D., Macieira-Coelho A. Interferon and cell division. V. Titration of the anticellular action of interferon preparations. Proc Soc Exp Biol Med. 1971 Sep;137(4):1258–1261. doi: 10.3181/00379727-137-35768. [DOI] [PubMed] [Google Scholar]
  8. HERMODSSON S., PHILIPSON L. A SENSITIVE METHOD FOR INTERFERON ASSAY. Proc Soc Exp Biol Med. 1963 Dec;114:574–579. doi: 10.3181/00379727-114-28735. [DOI] [PubMed] [Google Scholar]
  9. Huang K. Y., Donahoe R. M., Gordon F. B., Dressler H. R. Enhancement of phagocytosis by interferon-containing preparations. Infect Immun. 1971 Nov;4(5):581–588. doi: 10.1128/iai.4.5.581-588.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
  11. Lee S. H., O'Shaughnessy M. V., Rozee K. R., Kind L. S. Interferon induced growth depression in diploid and heteroploid human cells. Proc Soc Exp Biol Med. 1972 Apr;139(4):1438–1440. doi: 10.3181/00379727-139-36379. [DOI] [PubMed] [Google Scholar]
  12. Lindahl-Magnusson P., Leary P., Gresser I. Interferon and cell division. VI. Inhibitory effect of interferon on the multiplication of mouse embryo and mouse kidney cells in primary cultures. Proc Soc Exp Biol Med. 1971 Dec;138(3):1044–1050. doi: 10.3181/00379727-138-36047. [DOI] [PubMed] [Google Scholar]
  13. Lindahl-Magnusson P., Leary P., Gresser I. Interferon inhibits DNA synthesis induced in mouse lymphocyte suspensions by phytohaemagglutinin or by allogeneic cells. Nat New Biol. 1972 May 24;237(73):120–121. doi: 10.1038/newbio237120a0. [DOI] [PubMed] [Google Scholar]
  14. Lindahl P., Leary P., Gresser I. Enhancement by interferon of the specific cytotoxicity of sensitized lymphocytes. Proc Natl Acad Sci U S A. 1972 Mar;69(3):721–725. doi: 10.1073/pnas.69.3.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McWilliams M., Finkelstein M. S., Allen P. T., Giron D. J. Assay of chick interferons by the inhibition of viral ribonucleic acid synthesis. Appl Microbiol. 1971 May;21(5):959–961. doi: 10.1128/am.21.5.959-961.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Shaughnessy M. V., Lee S. H., Rozee K. R. Interferon inhibition of DNA synthesis and cell division. Can J Microbiol. 1972 Feb;18(2):145–151. doi: 10.1139/m72-024. [DOI] [PubMed] [Google Scholar]
  17. PAUCKER K., CANTELL K., HENLE W. Quantitative studies on viral interference in suspended L cells. III. Effect of interfering viruses and interferon on the growth rate of cells. Virology. 1962 Jun;17:324–334. doi: 10.1016/0042-6822(62)90123-x. [DOI] [PubMed] [Google Scholar]
  18. Pidot A. L. Dye uptake assay: an efficient and sensitive method for human interferon titration. Appl Microbiol. 1971 Oct;22(4):671–677. doi: 10.1128/am.22.4.671-677.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SREEVALSAN T., LOCKART R. Z., Jr A comparison of methods for interferon assay. Virology. 1962 May;17:207–208. doi: 10.1016/0042-6822(62)90102-2. [DOI] [PubMed] [Google Scholar]
  20. Steward W. E., 2nd, Gosser L. B., Lockart R. Z., Jr The effect of priming with interferon on interferon production by two lines of L cells. J Gen Virol. 1972 Apr;15(1):85–87. doi: 10.1099/0022-1317-15-1-85. [DOI] [PubMed] [Google Scholar]
  21. Stewart W. E., 2nd, De Clercq E., Billiau A., Desmyter J., De Somer P. Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic-polyribocytidylic acid. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1851–1854. doi: 10.1073/pnas.69.7.1851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stewart W. E., 2nd, De Clercq E., De Somer P. Recovery of cell-bound interferon. J Virol. 1972 Oct;10(4):707–712. doi: 10.1128/jvi.10.4.707-712.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stewart W. E., 2nd, Gosser L. B., Lockart R. Z., Jr Priming: a nonantiviral function of interferon. J Virol. 1971 Jun;7(6):792–801. doi: 10.1128/jvi.7.6.792-801.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tilles J. G., Finland M. Microassay for human and chick cell interferons. Appl Microbiol. 1968 Nov;16(11):1706–1707. doi: 10.1128/am.16.11.1706-1707.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WAGNER R. R. Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology. 1961 Mar;13:323–337. doi: 10.1016/0042-6822(61)90152-0. [DOI] [PubMed] [Google Scholar]
  26. Yoshino K., Morishima T. A simple and sensitive method for assay of chick interferon. Proc Soc Exp Biol Med. 1970 Dec;135(3):695–699. doi: 10.3181/00379727-135-35123. [DOI] [PubMed] [Google Scholar]
  27. Youngner J. S., Hallum J. V. Inhibition of induction of interferon synthesis in L-cells pretreated with interferon. Virology. 1969 Mar;37(3):473–475. doi: 10.1016/0042-6822(69)90231-1. [DOI] [PubMed] [Google Scholar]

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