Abstract
Bacteriophage T4 was grown in the presence of labeled uridine. The deoxyribonucleic acid (DNA) of the phage was shown to contain covalently attached ribonucleotides. The label appears not to be internal in the DNA strands. Presumably, it is at the ends of the DNA strands and this may be related to DNA initiation.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- APOSHIAN H. V., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem. 1962 Feb;237:519–525. [PubMed] [Google Scholar]
- Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
- Bazzicalupo P., Tocchini-Valentini G. P. Curing of an Escherichia coli episome by rifampicin (acridine orange-F + -F - -Hfr-lac). Proc Natl Acad Sci U S A. 1972 Feb;69(2):298–300. doi: 10.1073/pnas.69.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bremer H., Konrad M. W., Gaines K., Stent G. S. Direction of chain growth in enzymic RNA synthesis. J Mol Biol. 1965 Sep;13(2):540–553. doi: 10.1016/s0022-2836(65)80116-4. [DOI] [PubMed] [Google Scholar]
- Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHAMPE S. P., BENZER S. Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA. Proc Natl Acad Sci U S A. 1962 Apr 15;48:532–546. doi: 10.1073/pnas.48.4.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang L. M., Bollum F. J. A chemical model for transcriptional initiation of DNA replication. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354–1360. doi: 10.1016/s0006-291x(72)80124-4. [DOI] [PubMed] [Google Scholar]
- Chao J., Chao L., Speyer J. The inhibition of f2 RNA directed E. coli protein synthesis by poly rI-rC in vitro. Biochem Biophys Res Commun. 1971 Nov;45(4):1096–1102. doi: 10.1016/0006-291x(71)90450-5. [DOI] [PubMed] [Google Scholar]
- Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
- Colby C., Chamberlin M. J. The specificity of interferon induction in chick embryo cells by helical RNA. Proc Natl Acad Sci U S A. 1969 May;63(1):160–167. doi: 10.1073/pnas.63.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delius H., Howe C., Kozinski A. W. Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frankel F. R. DNA replication after T4 infection. Cold Spring Harb Symp Quant Biol. 1968;33:485–493. doi: 10.1101/sqb.1968.033.01.056. [DOI] [PubMed] [Google Scholar]
- Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
- Haskell E. H., Daverin C. I. Pre-fork synthesis: a model for DNA replication. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1065–1071. doi: 10.1073/pnas.64.3.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston J. H., Richmond M. H. The increased rate of loss of penicillinase plasmids from Staphylococcus aureus in the presence of rifampicin. J Gen Microbiol. 1970 Jan;60(1):137–139. doi: 10.1099/00221287-60-1-137. [DOI] [PubMed] [Google Scholar]
- Kleinschmidt W. J., Douthart R. J., Murphy E. B. Interferon production by T4 coliphage. Nature. 1970 Oct 3;228(5266):27–30. doi: 10.1038/228027a0. [DOI] [PubMed] [Google Scholar]
- Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEE-HUANG S., CAVALIERI L. F. POLYRIBONUCLEOTIDES AS TEMPLATES FOR POLYDEOXYRIBONUCLEOTIDES. Proc Natl Acad Sci U S A. 1963 Dec;50:1116–1122. doi: 10.1073/pnas.50.6.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lark K. G. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T. J Mol Biol. 1972 Feb 28;64(1):47–60. doi: 10.1016/0022-2836(72)90320-8. [DOI] [PubMed] [Google Scholar]
- Levinson W., Bishop J. M., Quintrell N., Jackson J. Presence of DNA in Rous sarcoma virus. Nature. 1970 Sep 5;227(5262):1023–1025. doi: 10.1038/2271023a0. [DOI] [PubMed] [Google Scholar]
- Maitra U., Hurwitz H. The role of DNA in RNA synthesis, IX. Nucleoside triphosphate termini in RNA polymerase products. Proc Natl Acad Sci U S A. 1965 Sep;54(3):815–822. doi: 10.1073/pnas.54.3.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mosig G. A preferred origin and direction of bacteriophage T4 DNA replication. I. A gradient of allele frequencies in crosses between normal and small T4 particles. J Mol Biol. 1970 Nov 14;53(3):503–514. doi: 10.1016/0022-2836(70)90080-x. [DOI] [PubMed] [Google Scholar]
- Murray R. E., Mathews C. K. Addition of nucleotides to parental DNA early in infection by bacteriophage T4. J Mol Biol. 1969 Sep 14;44(2):233–248. doi: 10.1016/0022-2836(69)90172-7. [DOI] [PubMed] [Google Scholar]
- Riva S., Fietta A. M., Silvestri L. G., Romero E. Effect of rifampicin on expression of some episomal genes in E. coli. Nat New Biol. 1972 Jan 19;235(55):78–80. doi: 10.1038/newbio235078a0. [DOI] [PubMed] [Google Scholar]
- Rokutanda M., Rokutanda H., Green M., Fujinaga K., Ray R. K., Gurgo C. Formation of viral RNA-DNA hybrid molecules by the DNA polymerase of sarcoma-leukaemia viruses. Nature. 1970 Sep 5;227(5262):1026–1028. doi: 10.1038/2271026a0. [DOI] [PubMed] [Google Scholar]
- Ríman J., Beaudreau G. S. Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus. Nature. 1970 Oct 31;228(5270):427–430. doi: 10.1038/228427a0. [DOI] [PubMed] [Google Scholar]
- STREISINGER G., EDGAR R. S., DENHARDT G. H. CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP. Proc Natl Acad Sci U S A. 1964 May;51:775–779. doi: 10.1073/pnas.51.5.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STRETTON A. O., BRENNER S. MOLECULAR CONSEQUENCES OF THE AMBER MUTATION AND ITS SUPPRESSION. J Mol Biol. 1965 Jun;12:456–465. doi: 10.1016/s0022-2836(65)80268-6. [DOI] [PubMed] [Google Scholar]
- Silverstein S., Billen D. Transcription: role in the initiation and replication of DNA synthesis in Escherichia coli and phiX174. Biochim Biophys Acta. 1971 Oct;247(3):383–390. doi: 10.1016/0005-2787(71)90023-2. [DOI] [PubMed] [Google Scholar]
- Speyer J. F., Rosenberg D. The function of T4 DNA polymerase. Cold Spring Harb Symp Quant Biol. 1968;33:345–350. doi: 10.1101/sqb.1968.033.01.040. [DOI] [PubMed] [Google Scholar]
- Streisinger G., Emrich J., Stahl M. M. Chromosome structure in phage t4, iii. Terminal redundancy and length determination. Proc Natl Acad Sci U S A. 1967 Feb;57(2):292–295. doi: 10.1073/pnas.57.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugino A., Hirose S., Okazaki R. RNA-linked nascent DNA fragments in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863–1867. doi: 10.1073/pnas.69.7.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THOMAS C. A., Jr, MACHATTIE L. A. CIRCULAR T2 DNA MOLECULES. Proc Natl Acad Sci U S A. 1964 Nov;52:1297–1301. doi: 10.1073/pnas.52.5.1297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tytell A. A., Lampson G. P., Field A. K., Hilleman M. R. Inducers of interferon and host resistance. 3. Double-stranded RNA from reovirus type 3 virions (reo 3-RNA). Proc Natl Acad Sci U S A. 1967 Oct;58(4):1719–1722. doi: 10.1073/pnas.58.4.1719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verma I. M., Meuth N. L., Bromfeld E., Manly K. F., Baltimore D. Covalently linked RNA-DNA molecule as initial product of RNA tumour virus DNA polymerase. Nat New Biol. 1971 Sep 29;233(39):131–134. doi: 10.1038/newbio233131a0. [DOI] [PubMed] [Google Scholar]
- Wells R. D., Flügel R. M., Larson J. E., Schendel P. F., Sweet R. W. Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli, and Micrococcus luteus. Biochemistry. 1972 Feb 15;11(4):621–629. doi: 10.1021/bi00754a025. [DOI] [PubMed] [Google Scholar]
- Werner R. Initiation and propagation of growing points in the DNA of phage T4. Cold Spring Harb Symp Quant Biol. 1968;33:501–507. doi: 10.1101/sqb.1968.033.01.058. [DOI] [PubMed] [Google Scholar]
- Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]