Abstract
A Sendai virus-induced transcriptase-template complex was isolated from the cytoplasm of infected cells by combined sedimentation and isopycnic centrifugation. This transcriptive complex banded at 1.27 g/cm3 in D2O-sucrose gradients. It contained two polypeptides, the viral nucleocapsid structure unit (molecular weight, 60,000) and the largest virion polypeptide (molecular weight, 75,000). The buoyant density, chemical composition, and electron microscopic appearance of the transcriptive complex indicate a structure like that of viral nucleocapsids.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blair C. D., Robinson W. S. Replication of Sendai virus. II. Steps in virus assembly. J Virol. 1970 May;5(5):639–650. doi: 10.1128/jvi.5.5.639-650.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Content J., Duesberg P. H. Electrophoretic distribution of the proteins and glycoproteins of influenza virus and Sendai virus. J Virol. 1970 Dec;6(6):707–716. doi: 10.1128/jvi.6.6.707-716.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darlington R. W., Portner A., Kingsbury D. W. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells. J Gen Virol. 1970 Dec;9(3):169–177. doi: 10.1099/0022-1317-9-3-169. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- Finch J. T., Gibbs A. J. Observations on the structure of the nucleocapsids of some paramyxoviruses. J Gen Virol. 1970 Jan;6(1):141–150. doi: 10.1099/0022-1317-6-1-141. [DOI] [PubMed] [Google Scholar]
- Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchinson J. E., Mahy B. W. RNA polymerase activity in virions of parainfluenza 1 virus (Sendai). Arch Gesamte Virusforsch. 1972;37(2):203–210. doi: 10.1007/BF01268002. [DOI] [PubMed] [Google Scholar]
- Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mahy B. W., Hutchinson J. E., Barry R. D. Ribonucleic acid polymerase induced in cells infected with Sendai virus. J Virol. 1970 Jun;5(6):663–671. doi: 10.1128/jvi.5.6.663-671.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Choppin P. W. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. doi: 10.1128/jvi.7.1.47-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEVILLE D. M., Jr The isolation of a cell membrane fraction from rat liver. J Biophys Biochem Cytol. 1960 Oct;8:413–422. doi: 10.1083/jcb.8.2.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
- Portner A., Kingsbury D. W. Identification of transcriptive and replicative intermediates in Sendai virus-infected cells. Virology. 1972 Mar;47(3):711–725. doi: 10.1016/0042-6822(72)90561-2. [DOI] [PubMed] [Google Scholar]
- Robinson W. S. Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid. J Virol. 1971 Jul;8(1):81–86. doi: 10.1128/jvi.8.1.81-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scholtissek C., Rott R. Ribonucleic acid nucleotidyl transferase induced in chick fibroblasts after infection with Newcastle disease virus. J Gen Virol. 1969 Jun;4(4):565–570. doi: 10.1099/0022-1317-4-4-565. [DOI] [PubMed] [Google Scholar]
- Stone H. O., Portner A., Kingsbury D. W. Ribonucleic acid transcriptases in Sendai Virions and infected cells. J Virol. 1971 Aug;8(2):174–180. doi: 10.1128/jvi.8.2.174-180.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]