Abstract
The core of the herpes simplex virion consists of an electron-dense toroidal structure 50 nm high, with an inside diameter of 18 nm and an outside diameter of 70 nm, penetrated by a less dense cylindrical mass. The toroid contains deoxyribonucleic acid (DNA), as evident from the observation that uranyl ion staining is removed by ethylenediaminetetraacetic acid under conditions which result in the extraction of uranyl ions bound to nuclear DNA. Studies on negatively stained preparations of purified capsids suggest that the toroid consists of DNA arranged as if it were spooled around the cylindrical mass.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Becker Y., Dym H., Sarov I. Herpes simplex virus DNA. Virology. 1968 Oct;36(2):184–192. doi: 10.1016/0042-6822(68)90135-9. [DOI] [PubMed] [Google Scholar]
- Bernhard W. A new staining procedure for electron microscopical cytology. J Ultrastruct Res. 1969 May;27(3):250–265. doi: 10.1016/s0022-5320(69)80016-x. [DOI] [PubMed] [Google Scholar]
- EPSTEIN M. A. Observations on the fine structure of mature herpes simplex virus and on the composition of its nucleoid. J Exp Med. 1962 Jan 1;115:1–12. doi: 10.1084/jem.115.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibson W., Roizman B. Compartmentalization of spermine and spermidine in the herpes simplex virion. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2818–2821. doi: 10.1073/pnas.68.11.2818. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roizmab, Spring S. B., Schwar J. The herpesvirion and its precursors made in productively and in abortively infected cells. Fed Proc. 1969 Nov-Dec;28(6):1890–1898. [PubMed] [Google Scholar]
- WILDY P., RUSSELL W. C., HORNE R. W. The morphology of herpes virus. Virology. 1960 Oct;12:204–222. doi: 10.1016/0042-6822(60)90195-1. [DOI] [PubMed] [Google Scholar]
- Zambernard J., Vatter A. E. The fine structural cytochemistry of virus particles found in renal tumors of leopard frogs. I. An enzymatic study of the viral nucleoid. Virology. 1966 Feb;28(2):318–324. doi: 10.1016/0042-6822(66)90155-3. [DOI] [PubMed] [Google Scholar]