Abstract
The temperature dependence of the virion-associated polymerase activity of six temperature-sensitive (ts) mutants of vesicular stomatitis virus (tsW10, 11, 14, 16B, 28, and 29) has been examined in vitro and compared to the heat-resistant parent (HR). The polymerase of five of the mutants (tsW10, 11, 14, 16B, and 28) appears to be significantly more ts than that of HR. Because certain pairs of these five mutants can complement each other's in vitro polymerase activity, it appears that in vitro some components involved in the polymerase of one virion can be utilized by another virion. Examination of 19 revertants of tsW11 and tsW16B which had regained their ability to replicate at 38 C showed that their in vitro polymerase activity had also become less ts. Furthermore, it was found that the pairs of mutants which showed in vitro complementation of polymerase activity at 38 C were those which had shown complementation in yielding infectious progeny in mixedly infected cells. These two observations suggest that the ts behavior of the in vitro polymerase activity of the five mutants is related to their inability to replicate at the nonpermissive temperature.
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Selected References
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- Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cormack D. V., Holloway A. F., Wong P. K., Cairns J. E. Temperature-sensitive mutants of vesicular stomatitis virus. II. Evidence of defective polymerase. Virology. 1971 Sep;45(3):824–826. doi: 10.1016/0042-6822(71)90205-4. [DOI] [PubMed] [Google Scholar]
- Holloway A. F., Wong P. K., Cormack D. V. Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus. Virology. 1970 Dec;42(4):917–926. doi: 10.1016/0042-6822(70)90340-5. [DOI] [PubMed] [Google Scholar]
- Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
- Pringle C. R., Duncan I. B. Preliminary physiological characterization of temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1971 Jul;8(1):56–61. doi: 10.1128/jvi.8.1.56-61.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pringle C. R. Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethyl methane sulfonate. J Virol. 1970 May;5(5):559–567. doi: 10.1128/jvi.5.5.559-567.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong P. K., Holloway A. F., Cormack D. V. A search for recombination between temperature-sensitive mutants of vesicular stomatitis virus. J Gen Virol. 1971 Dec;13(3):477–479. doi: 10.1099/0022-1317-13-3-477. [DOI] [PubMed] [Google Scholar]