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. 1972 Dec;10(6):1220–1227. doi: 10.1128/jvi.10.6.1220-1227.1972

Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

Jen Yeh 1, Yuzo Iwasaki 1
PMCID: PMC356604  PMID: 4118868

Abstract

Nucleocapsids from subacute sclerosing panencephalitis (SSPE) virus-infected CV-1 cells were concentrated by differential centrifugation employing sucrose cushion techniques and further purified by centrifugation through a linear CsCl density gradient. The bouyant density of 3H-uridine-labeled nucleocapsids in CsCl was found to be 1.31 g/cm3. Ultraviolet absorption spectra of the purified SSPE nucleocapsid showed an absorption maximum at 260 to 265 nm and a 280/260 ratio that corresponded to a nucleic acid content of approximately 4.3%. Negatively stained preparations of SSPE nucleocapsids were found to have a width of 18 ± 1 nm, a periodicity of 5 to 6 nm, and a length between 1-1.4 μm, with the greatest number at 1.3 μm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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