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. 1973 Aug;12(2):218–225. doi: 10.1128/jvi.12.2.218-225.1973

Hydroxyapatite Chromatography and Formamide Denaturation of Adenovirus DNA

Clark Tibbetts 1, Katarina Johansson 1, Lennart Philipson 1
PMCID: PMC356615  PMID: 4355929

Abstract

Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (Tm) of double-stranded DNA in solution or bound to hydroxyapatite. The Tm of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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