Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1973 Aug;12(2):226–229. doi: 10.1128/jvi.12.2.226-229.1973

Penetration into Host Cells of Naked, Partially Injected (Post-FST) DNA of Bacteriophage T5

B Labedan 1, J Legault-Demare 1
PMCID: PMC356616  PMID: 4583885

Abstract

The experiments presented here suggest that the naked post-FST DNA, attached to the bacteria after a centrifugation at 6,000 × g of Escherichia coli-T5 complexes arrested at the FST stage, can be injected into the host cell if protein synthesis is allowed. This results from the observation that the production of infectious centers by this naked DNA is sensitive to DNase or moderate shearing treatments. The process of injection seems to be the same as that for DNA normally rolled in an adsorbed capsid.

Full text

PDF
226

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckman L. D., Hoffman M. S., McCorquodale D. J. Pre-early proteins of bacteriophage T5: structure and function. J Mol Biol. 1971 Dec 28;62(3):551–564. doi: 10.1016/0022-2836(71)90155-0. [DOI] [PubMed] [Google Scholar]
  2. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5. J Virol. 1971 May;7(5):612–618. doi: 10.1128/jvi.7.5.612-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LANNI Y. T. Invasion by bacteriophage T5. II. Dissociation of calcium-independent and calcium-dependent processes. Virology. 1960 Apr;10:514–529. doi: 10.1016/0042-6822(60)90133-1. [DOI] [PubMed] [Google Scholar]
  4. LANNI Y. T. Invasion by bacteriophage T5. III. Stages revealed by changes in susceptibility of early complexes to abortive infection. Virology. 1961 Oct;15:127–135. doi: 10.1016/0042-6822(61)90229-x. [DOI] [PubMed] [Google Scholar]
  5. Labedan B., Crochet M., Legault-Demare J., Stevens B. J. Location of the first step transfer fragment and single-strand interruptions in T5stO bacteriophage DNA. J Mol Biol. 1973 Apr 5;75(2):213–234. doi: 10.1016/0022-2836(73)90017-x. [DOI] [PubMed] [Google Scholar]
  6. Lanni Y. T. DNA transfer from phage T5 to host cells: dependence on intercurrent protein synthesis. Proc Natl Acad Sci U S A. 1965 May;53(5):969–973. doi: 10.1073/pnas.53.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lanni Y. T. First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968 Sep;32(3):227–242. doi: 10.1128/br.32.3.227-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lanni Y. T., Lanni F., Tevethia M. J. Bacteriophage T5 chromosome fractionation: genetic specificity of a DNA fragment. Science. 1966 Apr 8;152(3719):208–210. doi: 10.1126/science.152.3719.208. [DOI] [PubMed] [Google Scholar]
  9. Lanni Y. Functions of two genes in the first-step-transfer DNA of bacteriophage T5. J Mol Biol. 1969 Aug 28;44(1):173–183. doi: 10.1016/0022-2836(69)90412-4. [DOI] [PubMed] [Google Scholar]
  10. MCCORQUODALE D. J., LANNI Y. T. MOLECULAR ASPECTS OF DNA TRANSFER FROM PHAGE T5 TO HOST CELLS. I. CHARACTERIZATION OF FIRST-STEP-TRANSFER MATERIAL. J Mol Biol. 1964 Oct;10:10–18. doi: 10.1016/s0022-2836(64)80023-1. [DOI] [PubMed] [Google Scholar]
  11. McCorquodale D. J., Lanni Y. T. Patterns of protein synthesis in Escherichia coli infected by amber mutants in the first-step-transfer DNA of T5. J Mol Biol. 1970 Feb 28;48(1):133–143. doi: 10.1016/0022-2836(70)90224-x. [DOI] [PubMed] [Google Scholar]
  12. Simon L. D., Anderson T. F. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. I. Attachment and penetration. Virology. 1967 Jun;32(2):279–297. doi: 10.1016/0042-6822(67)90277-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES