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. 1973 Aug;12(2):320–327. doi: 10.1128/jvi.12.2.320-327.1973

Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells

Robert H Bussell a,1, William S Robinson a
PMCID: PMC356626  PMID: 4355934

Abstract

A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 × 105. MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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