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. 1973 Aug;12(2):360–366. doi: 10.1128/jvi.12.2.360-366.1973

Effect of Viral Double-Stranded RNA on Mammalian Cells in Culture: Cytotoxicity Under Conditions Preventing Viral Replication and Protein Synthesis

B Cordell-Stewart a,1, Milton W Taylor a
PMCID: PMC356631  PMID: 4795868

Abstract

Noninfectious bovine enterovirus double-stranded RNA induces cytopathic effects when added to mammalian cells in culture. This is demonstrated by 51Cr release from prelabeled murine lymphoma cells and trypan blue uptake. Also, the induction of cell death by this viral RNA occurs in the presence of inhibitors of protein synthesis (cycloheximide and puromycin). The possible role and mechanism of viral, double-stranded RNA as a cytopathic agent in virally infected cells are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BABLANIAN R., EGGERS H. J., TAMM I. STUDIES ON THE MECHANISM OF POLIOVIRUS-INDUCED CELL DAMAGE. I. THE RELATION BETWEEN POLIOVIRUS,-INDUCED METABOLIC AND MORPHOLOGICAL ALTERATIONS IN CULTURED CELLS. Virology. 1965 May;26:100–113. doi: 10.1016/0042-6822(65)90030-9. [DOI] [PubMed] [Google Scholar]
  2. BABLANIAN R., EGGERS H. J., TAMM I. STUDIES ON THE MECHANISM OF POLIOVIRUS-INDUCED CELL DAMAGE. II. THE RELATION BETWEEN POLIOVIRUS GROWTH AND VIRUS-INDUCED MORPHOLOGICAL CHANGES IN CELLS. Virology. 1965 May;26:114–121. doi: 10.1016/0042-6822(65)90031-0. [DOI] [PubMed] [Google Scholar]
  3. BALTIMORE D., FRANKLIN R. M., CALLENDER J. MENGOVIRUS-INDUCED INHIBITION OF HOST RIBONUCLEIC ACID AND PROTEIN SYNTHESIS. Biochim Biophys Acta. 1963 Nov 22;76:425–430. [PubMed] [Google Scholar]
  4. Bablanian R. Depression of macromolecular synthesis in cells infected with guanidine-dependent poliovirus under restrictive conditions. Virology. 1972 Jan;47(1):255–259. doi: 10.1016/0042-6822(72)90260-7. [DOI] [PubMed] [Google Scholar]
  5. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  6. Bishop J. M., Koch G. Purification and characterization of poliovirus-induced infectious double-stranded ribonucleic acid. J Biol Chem. 1967 Apr 25;242(8):1736–1743. [PubMed] [Google Scholar]
  7. Bishop J. M., Summers D. F., Levintow L. Characterization of ribonuclease-resistant RNA from poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1273–1281. doi: 10.1073/pnas.54.4.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  9. Canty T. G., Wunderlich J. R. Quantitative in vitro assay of cytotoxic cellular immunity. J Natl Cancer Inst. 1970 Oct;45(4):761–772. [PubMed] [Google Scholar]
  10. Cordell-Stewart B., Taylor M. W. Effect of double-stranded viral RNA on mammalian cells in culture. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1326–1330. doi: 10.1073/pnas.68.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cordell-Stewart B., Taylor M. W. Effect of viral double-stranded RNA on protein synthesis in intact cells. J Virol. 1973 Feb;11(2):232–237. doi: 10.1128/jvi.11.2.232-237.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
  13. Ehrenfeld E., Hunt T. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates. Proc Natl Acad Sci U S A. 1971 May;68(5):1075–1078. doi: 10.1073/pnas.68.5.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hunt T., Ehrenfeld E. Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis. Nat New Biol. 1971 Mar 17;230(11):91–94. doi: 10.1038/newbio230091a0. [DOI] [PubMed] [Google Scholar]
  15. Koschel K. Freisetzung von 51 Chrom aus markierten HeLa-Zellen nach Poliovirus-Infektion. Z Naturforsch B. 1971 Sep;26(9):929–933. [PubMed] [Google Scholar]
  16. Koschel K., Täuber H., Wecker E. Early functions of poliovirus. I. The inhibition of cellular protein synthesis in HeLa cells after infection with active and inactivated poliovirus. Z Naturforsch B. 1971 Aug;26(8):798–803. doi: 10.1515/znb-1971-0813. [DOI] [PubMed] [Google Scholar]
  17. Latham H., Darnell J. E. Entrance of mRNA into HeLa cell cytoplasm in puromycin-treated cells. J Mol Biol. 1965 Nov;14(1):13–22. doi: 10.1016/s0022-2836(65)80225-x. [DOI] [PubMed] [Google Scholar]
  18. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  19. Penman S., Summers D. Effects on host cell metabolism following synchronous infection with poliovirus. Virology. 1965 Dec;27(4):614–620. doi: 10.1016/0042-6822(65)90187-x. [DOI] [PubMed] [Google Scholar]
  20. Polatnick J., Arlinghaus R. B., Graves J. H., Cowan K. M. Inhibition of cell-free foot-and-mouth disease virus-ribonucleic acid synthesis by antibody. Virology. 1967 Apr;31(4):609–615. doi: 10.1016/0042-6822(67)90189-4. [DOI] [PubMed] [Google Scholar]
  21. Stern R., Friedman R. M. Ribonucleic acid synthesis in animal cells in the presence of actinomycin. Biochemistry. 1971 Sep 28;10(20):3635–3645. doi: 10.1021/bi00796a001. [DOI] [PubMed] [Google Scholar]
  22. Stewart W. E., 2nd, De Clercq E., Billiau A., Desmyter J., De Somer P. Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic-polyribocytidylic acid. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1851–1854. doi: 10.1073/pnas.69.7.1851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wall R., Taylor M. W. Mengovirus RNA synthesis in productive and restrictive cell lines. Virology. 1970 Sep;42(1):78–86. doi: 10.1016/0042-6822(70)90240-0. [DOI] [PubMed] [Google Scholar]

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