Abstract
Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with 3H-uridine and 3H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm3. The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 × 106.
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Selected References
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- Bader J. P., Steck T. L. Analysis of the ribonucleic acid of murine leukemia virus. J Virol. 1969 Oct;4(4):454–459. doi: 10.1128/jvi.4.4.454-459.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darnell M. B., Plagemann P. G. Physical properties of lactic dehydrogenase-elevating virus and its ribonucleic acid. J Virol. 1972 Nov;10(5):1082–1085. doi: 10.1128/jvi.10.5.1082-1085.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Du Buy H. G., Johnson M. L. Some properties of the lactic dehydrogenase agent of mice. J Exp Med. 1965 Sep 1;122(3):587–600. doi: 10.1084/jem.122.3.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Melnick J. L. Classification and nomenclature of animal viruses, 1971. Prog Med Virol. 1971;13:462–484. [PubMed] [Google Scholar]
- NOTKINS A. L. LACTIC DEHYDROGENASE VIRUS. Bacteriol Rev. 1965 Jun;29:143–160. doi: 10.1128/br.29.2.143-160.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOTKINS A. L. RECOVERY OF AN INFECTIOUS RIBONUCLEIC ACID FROM THE LACTIC DEHYDROGENASE AGENT BY TREATMENT WITH ETHER. Virology. 1964 Apr;22:563–567. doi: 10.1016/0042-6822(64)90077-7. [DOI] [PubMed] [Google Scholar]
- Notkins A. L. Enzymatic and immunologic alterations in mice infected with lactic dehydrogenase virus. Am J Pathol. 1971 Sep;64(3):733–746. [PMC free article] [PubMed] [Google Scholar]
- Notkins A. L., Rosenthal J., Johnson B. Rate-zonal centrifugation of herpes simplex virus-antibody complexes. Virology. 1971 Jan;43(1):321–325. doi: 10.1016/0042-6822(71)90253-4. [DOI] [PubMed] [Google Scholar]
- Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
- Rowson K. E., Parr I. B., Alper T. Radiation target size of Riley virus. Virology. 1968 Sep;36(1):157–159. [PubMed] [Google Scholar]
- Yamazaki S., Notkins A. L. Inhibition of replication of lactic dehydrogenase virus by actinomycin. J Virol. 1973 Apr;11(4):473–478. doi: 10.1128/jvi.11.4.473-478.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
