Abstract
Infection of Bacillus subtilis by PBS2 phage, whose DNA contains uracil instead of thymine, is relatively unaffected by low concentrations of nalidixic acid which severely inhibit B. subtilis DNA synthesis. High concentrations of nalidixic acid do inhibit PBS2 DNA synthesis, but more severely reduce the burst size of PBS2 infections. Hydroxyurea blocks PBS2 DNA synthesis, preventing progeny phage production.
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Selected References
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- Baird J. P., Bourguignon G. J., Sternglanz R. Effect of nalidixic acid on the growth of deoxyribonucleic acid bacteriophages. J Virol. 1972 Jan;9(1):17–21. doi: 10.1128/jvi.9.1.17-21.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bazill G. W., Karamata D. Temperature-sensitive mutants of B. subtilis defective in deoxyribonucleotide synthesis. Mol Gen Genet. 1972;117(1):19–29. [PubMed] [Google Scholar]
- Cook T. M., Brown K. G., Boyle J. V., Goss W. A. Bactericidal action of nalidixic acid on Bacillus subtilis. J Bacteriol. 1966 Nov;92(5):1510–1514. doi: 10.1128/jb.92.5.1510-1514.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gage L. P., Fujita D. J. Effect of nalidixic acid on deoxyribonucleic acid synthesis in bacteriophage SPO1-infected Bacillus subtilis. J Bacteriol. 1969 Apr;98(1):96–103. doi: 10.1128/jb.98.1.96-103.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuhard J., Thomassen E. Turnover of the deoxyribonucleoside triphosphates in Escherichia coli 15 T during thymine starvation. Eur J Biochem. 1971 May 11;20(1):36–43. doi: 10.1111/j.1432-1033.1971.tb01359.x. [DOI] [PubMed] [Google Scholar]
- Pedrini A. M., Geroldi D., Falaschi A. Nalidixic acid does not inhibit bacterial transformation. Mol Gen Genet. 1972;116(1):91–94. doi: 10.1007/BF00334264. [DOI] [PubMed] [Google Scholar]
- Pedrini A. M., Geroldi D., Siccardi A., Falaschi A. Studies on the mode of action of nalidixic acid. Eur J Biochem. 1972 Feb 15;25(2):359–365. doi: 10.1111/j.1432-1033.1972.tb01704.x. [DOI] [PubMed] [Google Scholar]
- Price A. R., Cook S. J. New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2. J Virol. 1972 Apr;9(4):602–610. doi: 10.1128/jvi.9.4.602-610.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price A. R., Fogt S. M. Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis. J Biol Chem. 1973 Feb 25;248(4):1372–1380. [PubMed] [Google Scholar]
- Price A. R., Fogt S. M. Resistance of bacteriophage PBS2 infection to 6-(p-hydroxyphenylazo)-uracil, an inhibitor of Bacillus subtilis deoxyribonucleic acid synthesis. J Virol. 1973 Feb;11(2):338–340. doi: 10.1128/jvi.11.2.338-340.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price A. R., Frabotta M. Resistance of bacteriophage PBS2 infection to rifampicin, an inhibitor of Bacillus subtilis RNA synthesis. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1578–1585. doi: 10.1016/0006-291x(72)90894-7. [DOI] [PubMed] [Google Scholar]
- Sinha N. K., Snustad D. P. Mechanism of inhibition of deoxyribonucleic acid synthesis in Escherichia coli by hydroxyurea. J Bacteriol. 1972 Dec;112(3):1321–1324. doi: 10.1128/jb.112.3.1321-1334.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taketo A., Watanabe H. Effect of nalidixic acid on the growth of bacterial viruses. J Biochem. 1967 Apr;61(4):520–522. doi: 10.1093/oxfordjournals.jbchem.a128579. [DOI] [PubMed] [Google Scholar]
- Warner H. R., Hobbs M. D. Effect of hydroxyurea on replication of bacteriophage T4 in Escherichia coli. J Virol. 1969 Mar;3(3):331–336. doi: 10.1128/jvi.3.3.331-336.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]