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Journal of Virology logoLink to Journal of Virology
. 1973 Sep;12(3):421–430. doi: 10.1128/jvi.12.3.421-430.1973

Correlation Between the Antiviral Effect of Interferon Treatment and the Inhibition of In Vitro mRNA Translation in Noninfected L cells

E Falcoff 1,2, R Falcoff 1,2, B Lebleu 1,2, M Revel 1,2
PMCID: PMC356647  PMID: 4355848

Abstract

When noninfected L-cell suspension cultures are treated with interferon (specific activities superior to 106 reference units per mg of protein), the cell-free cytoplasmic extracts obtained are inactive for the translation of exogenous natural mRNAs. The dose-response curve shows that comparable amounts of interferon are required to produce a 50% reduction of Mengo virus multiplication in vivo and Mengo RNA translation in vitro. With higher doses of interferon, Mengo RNA translation is completely abolished, while poly U translation and endogenous protein synthesis are only slightly affected. The inactivation of Mengo RNA translation is reversible; after removal of interferon, normal translation activity is regained together with the ability to support Mengo virus multiplication. Fractionation of the cell-free extracts shows that the effect is localized in the fraction which can be washed off the ribosomes by high salt. These results establish that interferon induces a block in genetic translation in noninfected L cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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