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. 1973 Sep;12(3):538–547. doi: 10.1128/jvi.12.3.538-547.1973

Control of Gene Function in Bacteriophage T4 IV. Post-Transcriptional Shutoff of Expression of Early Genes

Walter Sauerbier 1, Kathleen Hercules 1
PMCID: PMC356660  PMID: 4583805

Abstract

The selective and sequential shutoff of synthesis of early T4 proteins in bacteria infected with DNA-negative mutants is under the active control of one or more T4-induced proteins. Selective shutoff of synthesis of early T4 proteins is accompanied by a selective degradation of distinct species of T4 mRNA. We present circumstantial evidence that selective degradation of mRNA is the cause, and not the consequence, of selective termination of expression of early T4 genes. The mutation sp62 inactivates the shutoff mechanism and prevents the selective degradation of distinct species of T4 mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gamow R. I., Kozloff L. M. Chemically induced cofactor requirement for bacteriopage T4D. J Virol. 1968 May;2(5):480–487. doi: 10.1128/jvi.2.5.480-487.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Groner Y., Pollack Y., Berissi H., Revel M. Characterization of cistron specific factors for the initiation of messenger RNA translation in E. coli. FEBS Lett. 1972 Mar 15;21(2):223–228. doi: 10.1016/0014-5793(72)80142-x. [DOI] [PubMed] [Google Scholar]
  3. HALL B. D., NYGAARD A. P., GREEN M. H. CONTROL OF T2-SPECIFIC RNA SYNTHESIS. J Mol Biol. 1964 Jul;9:143–153. doi: 10.1016/s0022-2836(64)80096-6. [DOI] [PubMed] [Google Scholar]
  4. HILL R. F. A radiation-sensitive mutant of Escherichia coli. Biochim Biophys Acta. 1958 Dec;30(3):636–637. doi: 10.1016/0006-3002(58)90112-4. [DOI] [PubMed] [Google Scholar]
  5. Hosoda J., Levinthal C. Protein synthesis by Escherichia coli infected with bacteriophage T4D. Virology. 1968 Apr;34(4):709–727. doi: 10.1016/0042-6822(68)90092-5. [DOI] [PubMed] [Google Scholar]
  6. Lee-Huang S., Ochoa S. Messenger discriminating species of initiation factor F3. Nat New Biol. 1971 Dec 22;234(51):236–239. doi: 10.1038/newbio234236a0. [DOI] [PubMed] [Google Scholar]
  7. NATHANS D. PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS: INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINS. Proc Natl Acad Sci U S A. 1964 Apr;51:585–592. doi: 10.1073/pnas.51.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. RICHMOND M. H. The effect of amino acid analogues on growth and protein synthesis in microorganisms. Bacteriol Rev. 1962 Dec;26:398–420. doi: 10.1128/br.26.4.398-420.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sakiyama S., Buchanan J. M. Control of the synthesis of T4 phage deoxynucleotide kinase messenger ribonucleic acid in vivo. J Biol Chem. 1972 Dec 10;247(23):7806–7814. [PubMed] [Google Scholar]
  10. Sakiyama S., Buchanan J. M. In vitro synthesis of deoxynucleotide kinase programmed by bacteriophage "T4-RNA. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1376–1380. doi: 10.1073/pnas.68.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salser W., Bolle A., Epstein R. Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of the "early" RNA appear at different times and that some are "turned off" at late times. J Mol Biol. 1970 Apr 28;49(2):271–295. doi: 10.1016/0022-2836(70)90246-9. [DOI] [PubMed] [Google Scholar]
  12. Sauerbier W., Bräutigam A. R. A simple method for isolating RNA from bacteria. Biochim Biophys Acta. 1970 Jan 21;199(1):36–40. doi: 10.1016/0005-2787(70)90692-1. [DOI] [PubMed] [Google Scholar]
  13. Sauerbier W., Schweiger M., Herrlich P. Control of gene function in bacteriophage Tr. 3. Preventing the shutoff of early enzyme synthesis. J Virol. 1971 Nov;8(5):613–618. doi: 10.1128/jvi.8.5.613-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sippel A., Hartmann G. Mode of action of rafamycin on the RNA polymerase reaction. Biochim Biophys Acta. 1968 Mar 18;157(1):218–219. doi: 10.1016/0005-2787(68)90286-4. [DOI] [PubMed] [Google Scholar]
  15. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  16. Trimble R. B., Galivan J., Maley F. The temporal expression of T2r + bacteriophage genes in vivo and in vitro. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1659–1663. doi: 10.1073/pnas.69.7.1659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Young E. T., 2nd, van Houwe G. Control of synthesis of glucosyl transferase and lysozyme messengers after T4 infection. J Mol Biol. 1970 Aug;51(3):605–619. doi: 10.1016/0022-2836(70)90011-2. [DOI] [PubMed] [Google Scholar]

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