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. 1973 Sep;12(3):579–587. doi: 10.1128/jvi.12.3.579-587.1973

Precursor Protein for Newcastle Disease Virus

A C R Samson a,1, C F Fox a
PMCID: PMC356665  PMID: 4795832

Abstract

The course of viral protein synthesis during infection of chicken embryo fibroblasts with Newcastle disease virus (NDV) L. Kansas has been followed by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Of the three major virion polypeptide molecular weight classes, I (78,400 daltons), II (53,500 daltons), and III (37,600 daltons), only II, having the same electrophoretic mobility as nucleocapsid polypeptide, appears to be the cleavage product of a precursor polypeptide PII (64,800 daltons) detected in NDV-infected cells after brief labeling with radioactive amino acids. Nucleocapsids were isolated from NDV-infected cells which had been pulse-labeled with radioactive amino acids or pulse-labeled and further incubated with unlabeled amino acids. Gel electrophoretic analysis of proteins derived from nucleocapsids showed that an increase in the period of incubation with unlabeled amino acids resulted in an increase in the amount of radioactivity in nucleocapsid protein. Polypeptide PII was not detected as a transient component of the isolated nucleocapsid fraction. These results are consistent with two interpretations. The product of PII cleavage is (i) nucleocapsid polypeptide, or (ii) a nonvirion or minor envelope polypeptide having the same electrophoretic mobility as nucleocapsid polypeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander D. J., Reeve P. The proteins of Newcastle disease virus. 1. Structural proteins. Microbios. 1972 May-Jun;5(19):199–212. [PubMed] [Google Scholar]
  2. Alexander D. J., Reeve P. The proteins of Newcastle disease virus. 2. Virus-induced proteins. Microbios. 1972;5(20):247–257. [PubMed] [Google Scholar]
  3. Anker H. S. A solubilizable acrylamide gel for electrophoresis. FEBS Lett. 1970 Apr 16;7(3):293–293. doi: 10.1016/0014-5793(70)80185-5. [DOI] [PubMed] [Google Scholar]
  4. Bikel I., Duesberg P. H. Proteins of Newcastle disease virus and of the viral nucleocapsid. J Virol. 1969 Oct;4(4):388–393. doi: 10.1128/jvi.4.4.388-393.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans M. J., Kingsbury D. W. Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis. Virology. 1969 Apr;37(4):597–604. doi: 10.1016/0042-6822(69)90277-3. [DOI] [PubMed] [Google Scholar]
  6. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  7. Haslam E. A., Cheyne I. M., White D. O. The structural proteins of Newcastle disease virus. Virology. 1969 Sep;39(1):118–129. doi: 10.1016/0042-6822(69)90353-5. [DOI] [PubMed] [Google Scholar]
  8. Kaverin N. V. Delay in the incorporation of protein into virus nucleocapsid in Newcastle disease virus infected cells. J Gen Virol. 1972 Dec;17(3):337–341. doi: 10.1099/0022-1317-17-3-337. [DOI] [PubMed] [Google Scholar]
  9. Kiehn E. D., Holland J. J. Multiple protein components of mammalian cell membranes. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1370–1377. doi: 10.1073/pnas.61.4.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kingsbury D. W., Darlington R. W. Isolation and properties of Newcastle disease virus nucleocapsid. J Virol. 1968 Mar;2(3):248–255. doi: 10.1128/jvi.2.3.248-255.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lomniczi B., Meager A., Burke D. C. Virus RNA and protein synthesis in cells infected with different strains of Newcastle disease virus. J Gen Virol. 1971 Oct;13(1):111–120. doi: 10.1099/0022-1317-13-1-111. [DOI] [PubMed] [Google Scholar]
  14. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mountcastle W. E., Compans R. W., Choppin P. W. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. doi: 10.1128/jvi.7.1.47-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scheid A., Choppin P. W. Isolation and purification of the envelope proteins of Newcastle disease virus. J Virol. 1973 Feb;11(2):263–271. doi: 10.1128/jvi.11.2.263-271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schimke R. T., Doyle D. Control of enzyme levels in animal tissues. Annu Rev Biochem. 1970;39:929–976. doi: 10.1146/annurev.bi.39.070170.004433. [DOI] [PubMed] [Google Scholar]
  18. Shapiro S. C., Bratt M. S. Proteins of four biologically distinct strains of Newcastle disease virus. Proc Soc Exp Biol Med. 1971 Mar;136(3):834–838. doi: 10.3181/00379727-136-35375. [DOI] [PubMed] [Google Scholar]

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