Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1973 Sep;12(3):653–658. doi: 10.1128/jvi.12.3.653-658.1973

Mapping of Simian Virus 40 Early Functions on the Viral Chromosome

John F Morrow 1,2, Paul Berg 1,2, Thomas J Kelly Jr 1,2,1, Andrew M Lewis Jr 1,2
PMCID: PMC356674  PMID: 4355862

Abstract

The simian virus 40 (SV40) DNA segment in the nondefective adenovirus 2-SV40 hybrid, Ad2+ND4, is colinear with the segment between 0.11 and 0.59 SV40 fractional length from the site at which the R1 restriction endonuclease cleaves SV40 DNA. This specifies the region of the SV40 DNA molecule which induces the early SV40 antigens: U antigen, tumor specific transplantation antigen, and T antigen. A variant of Ad2+ND4, called Ad2+ND4del, was found which has a deletion of the DNA segment between 0.50 and 0.57 SV40 fractional length from the R1 endonuclease cleavage point.

Full text

PDF
653

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. Danna K., Nathans D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913–2917. doi: 10.1073/pnas.68.12.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henry P. H., Schnipper L. E., Samaha R. J., Crumpacker C. S., Lewis A. M., Jr, Levine A. S. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VI. Characterization of the DNA from five nondefective hybrid viruses. J Virol. 1973 May;11(5):665–671. doi: 10.1128/jvi.11.5.665-671.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly T. J., Jr, Lewis A. M., Jr Use of nondefective adenovirus-simian virus 40 hybrids for mapping the simian virus 40 genome. J Virol. 1973 Sep;12(3):643–652. doi: 10.1128/jvi.12.3.643-652.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Levin M. J., Crumpacker C. S., Lewis A. M., Jr, Oxman M. N., Henry P. H., Rowe W. P. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. II. Relationship of adenovirus 2 deoxyribonucleic acid and simian virus 40 deoxyribonucleic acid in the Ad2+ND genome. J Virol. 1971 Mar;7(3):343–351. doi: 10.1128/jvi.7.3.343-351.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Levine A. S., Levin M. J., Oxman M. N., Lewis A. M., Jr Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses. J Virol. 1973 May;11(5):672–681. doi: 10.1128/jvi.11.5.672-681.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lewis A. M., Jr, Levin M. J., Wiese W. H., Crumpacker C. S., Henry P. H. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lewis A. M., Jr, Rowe W. P. Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. J Virol. 1971 Feb;7(2):189–197. doi: 10.1128/jvi.7.2.189-197.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mertz J. E., Davis R. W. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3370–3374. doi: 10.1073/pnas.69.11.3370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morrow J. F., Berg P. Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3365–3369. doi: 10.1073/pnas.69.11.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mulder C., Delius H. Specificity of the break produced by restricting endonuclease R 1 in Simian virus 40 DNA, as revealed by partial denaturation mapping. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3215–3219. doi: 10.1073/pnas.69.11.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Patch C. T., Lewis A. M., Jr, Levine A. S. Evidence for a transcription-control region of Simian virus 40 in the adenovirus 2--Simian virus 40 hybrid, Ad2+ND 1 . Proc Natl Acad Sci U S A. 1972 Nov;69(11):3375–3379. doi: 10.1073/pnas.69.11.3375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sauer G., Kidwai J. R. The transcription of the SV40 genome in productively infected and transformed cells. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1256–1263. doi: 10.1073/pnas.61.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wolfson J., Dressler D. Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3054–3057. doi: 10.1073/pnas.69.10.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES