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. 1973 Oct;12(4):847–854. doi: 10.1128/jvi.12.4.847-854.1973

Bacteriophage T7 DNA Synthesis in Isolated DNA-Membrane Complexes

Melvin S Center 1
PMCID: PMC356703  PMID: 4591051

Abstract

A DNA-membrane complex isolated from Escherichia coli infected with bacteriophage T7 contains newly synthesized T7 DNA and the T7 DNA polymerase (gene 5 product). The DNA present in the complex appears to exist as a concatemer which contains single-strand breaks and possibly internal single-stranded regions (gaps). The complex is capable of synthesizing T7 DNA by using endogenous template, and part of the DNA is made by a semiconservative mechanism. A portion of the in vitro synthesized DNA sediments in alkaline sucrose as 10-11S material. This DNA is converted to a larger-molecular-weight material after treatment with T4 polynucleotide ligase and E. coli DNA polymerase I.

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Selected References

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  1. Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Becker A., Lyn G., Gefter M., Hurwitz J. The enzymatic repair of DNA, II. Characterization of phage-induced sealase. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1996–2003. doi: 10.1073/pnas.58.5.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Center M. S. Replicative intermediates of bacteriophage T7 deoxyribonucleic acid. J Virol. 1972 Jul;10(1):115–123. doi: 10.1128/jvi.10.1.115-123.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Center M. S., Richardson C. C. An endonuclease induced after infection of Escherichia coli with bacteriophage T7. I. Purification and properties of the enzyme. J Biol Chem. 1970 Dec 10;245(23):6285–6291. [PubMed] [Google Scholar]
  5. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  6. Dressler D., Wolfson J., Magazin M. Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7. Proc Natl Acad Sci U S A. 1972 Apr;69(4):998–1002. doi: 10.1073/pnas.69.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grippo P., Richardson C. C. Deoxyribonucleic acid polymerase of bacteriophage T7. J Biol Chem. 1971 Nov 25;246(22):6867–6873. [PubMed] [Google Scholar]
  8. Kelly T. J., Jr, Thomas C. A., Jr An intermediate in the replication of bacteriophage T7 DNA molecules. J Mol Biol. 1969 Sep 28;44(3):459–475. doi: 10.1016/0022-2836(69)90373-8. [DOI] [PubMed] [Google Scholar]
  9. LEHMAN I. R., NUSSBAUM A. L. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE). J Biol Chem. 1964 Aug;239:2628–2636. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Masamune Y., Frenkel G. D., Richardson C. C. A mutant of bacteriophage T7 deficient in polynucleotide ligase. J Biol Chem. 1971 Nov 25;246(22):6874–6879. [PubMed] [Google Scholar]
  12. Okazaki R., Sugimoto K., Okazaki T., Imae Y., Sugino A. DNA chain growth: in vivo and in vitro synthesis in a DNA polymerase-negative mutant of E. coli. Nature. 1970 Oct 17;228(5268):223–226. doi: 10.1038/228223a0. [DOI] [PubMed] [Google Scholar]
  13. Pacumbaba R. P., Center M. S. Association of replicating bacteriophage T7 DNA with bacterial membranes. J Virol. 1973 Oct;12(4):855–861. doi: 10.1128/jvi.12.4.855-861.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Richardson C. C. Enzymes in DNA metabolism. Annu Rev Biochem. 1969;38:795–840. doi: 10.1146/annurev.bi.38.070169.004051. [DOI] [PubMed] [Google Scholar]
  15. Ritchie D. A., Thomas C. A., Jr, MacHattie L. A., Wensink P. C. Terminal repetition in non-permuted T3 and T7 bacteriophage DNA molecules. J Mol Biol. 1967 Feb 14;23(3):365–376. doi: 10.1016/s0022-2836(67)80111-6. [DOI] [PubMed] [Google Scholar]
  16. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  17. Strätling W., Knippers R. Deoxyribonucleic acid synthesis in isolated DNA-membrane complexes. J Mol Biol. 1971 Nov 14;61(3):471–487. doi: 10.1016/0022-2836(71)90060-x. [DOI] [PubMed] [Google Scholar]
  18. Strätling W., Knippers R. Properties of the DNA synthesizing activity in DNA-membrane complexes from bacterial cell extracts. Eur J Biochem. 1971 Jun 11;20(3):330–339. doi: 10.1111/j.1432-1033.1971.tb01398.x. [DOI] [PubMed] [Google Scholar]
  19. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  20. Watson J. D. Origin of concatemeric T7 DNA. Nat New Biol. 1972 Oct 18;239(94):197–201. doi: 10.1038/newbio239197a0. [DOI] [PubMed] [Google Scholar]
  21. Weiss B., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021–1028. doi: 10.1073/pnas.57.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wolfson J., Dressler D., Magazin M. Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E. coli-DNA partial denaturation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):499–504. doi: 10.1073/pnas.69.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wolfson J., Dressler D. Regions of single-stranded DNA in the growing points of replicating bacteriophage T7 chromosomes. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2682–2686. doi: 10.1073/pnas.69.9.2682. [DOI] [PMC free article] [PubMed] [Google Scholar]

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