Abstract
Infection of Escherichia coli with bacteriophage T7 leads to the formation of an association between host membranes and newly synthesized T7 DNA. Evidence for this conclusion is suggested by the findings that replicating T7 DNA cosediments with host membranes in sucrose and cesium chloride density gradients. Furthermore, the sedimentation rate of T7 DNA is dependent on the integrity of membrane structure. Finally, an association between DNA and membranes can be demonstrated by electron microscope studies.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Center M. S. Bacteriophage T7 DNA synthesis in isolated DNA-membrane complexes. J Virol. 1973 Oct;12(4):847–854. doi: 10.1128/jvi.12.4.847-854.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Center M. S. Replicative intermediates of bacteriophage T7 deoxyribonucleic acid. J Virol. 1972 Jul;10(1):115–123. doi: 10.1128/jvi.10.1.115-123.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green E. W., Schaechter M. The mode of segregation of the bacterial cell membrane. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2312–2316. doi: 10.1073/pnas.69.8.2312. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jazwinski S. M., Marco R., Kornberg A. A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):205–209. doi: 10.1073/pnas.70.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
- Kelly T. J., Jr, Thomas C. A., Jr An intermediate in the replication of bacteriophage T7 DNA molecules. J Mol Biol. 1969 Sep 28;44(3):459–475. doi: 10.1016/0022-2836(69)90373-8. [DOI] [PubMed] [Google Scholar]
- Miller R. C., Jr Association of replicative T4 deoxyribonucleic acid and bacterial membranes. J Virol. 1972 Nov;10(5):920–924. doi: 10.1128/jvi.10.5.920-924.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miura T., Mizushima S. Separation by density gradient centrifugation of two types of membranes from spheroplast membrane of Escherichia coli K12. Biochim Biophys Acta. 1968 Jan 3;150(1):159–161. doi: 10.1016/0005-2736(68)90020-5. [DOI] [PubMed] [Google Scholar]
- Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
- Sadowski P. D., Kerr C. Degradation of Escherichia coli B deoxyribonucleic acid after infection with deoxyribonucleic acid-defective amber mutants of bacteriophage T7. J Virol. 1970 Aug;6(2):149–155. doi: 10.1128/jvi.6.2.149-155.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strätling W., Knippers R. Deoxyribonucleic acid synthesis in isolated DNA-membrane complexes. J Mol Biol. 1971 Nov 14;61(3):471–487. doi: 10.1016/0022-2836(71)90060-x. [DOI] [PubMed] [Google Scholar]
- Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]