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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Biochemistry. 2012 Nov 26;51(49):9911–9921. doi: 10.1021/bi301386q

Figure 4. Binding of activated Cry5B and Cry5B protoxin to upper phase glycolipids.

Figure 4

Upper phase glycolipids of C. elegans (C.e.) and P. pacificus (P.p.) were resolved by thin layer chromatography and stained with orcinol (a and g), and overlaid with elastase-activated Cry5B (b–f) or Cry5B protoxin (h–l) in the presence of 100 mM galactose (Gal, c, i), 100 mM glucose (Glc, d, j), 100 mM N-acetylglucosamine (GlcNAc, e, k), or 100 mM N-acetylgalactosamine (GalNAc, f, 1). The glycolipid band species are indicated to the left of panels b and h. This is a representative set from several experiments carried out.