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. 1973 Nov;12(5):1031–1033. doi: 10.1128/jvi.12.5.1031-1033.1973

Isolation and Characterization of a Bacteriophage of Arthrobacter globiformis

K H Einck 1,2,1, P A Pattee 1,2, J G Holt 1,2, C Hagedorn 1,2, J A Miller 1,2, D L Berryhill 1,2
PMCID: PMC356733  PMID: 4128824

Abstract

A bacteriophage which reproduces on Arthrobacter globiformis ATCC 8010 was isolated from soil. This bacteriophage, designated φAG8010, propagates either in soft agar or broth cultures of the host. Because of a slow adsorption rate, neither the latent period nor burst size was determined. The mature virion belongs to Bradley's group B and exhibits a hexagonal head measuring 69 nm (length) by 60 nm (width) attached to a sheathless tail 120 nm long. The buoyant density of the mature virion is 1.534 g/cm3. The mature virion contains double-stranded DNA with a buoyant density of 1.722 g/cm3 (equivalent to 63.3% G + C). Of 14 strains (representing 13 species) of Arthrobacter examined, including A. globiformis ATCC 4336, only A. globiformis ATCC 8010 supported replication of φAG8010.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berryhill D. L., Pattee P. A. Buoyant density analysis of staphylococcal bacteriophage 80 transducing particles. J Virol. 1969 Nov;4(5):804–806. doi: 10.1128/jvi.4.5.804-806.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Conn H. J., Bottcher E. J., Randall C. The Value of Bacteriophage in Classifying Certain Soil Bacteria. J Bacteriol. 1945 Apr;49(4):359–373. doi: 10.1128/jb.49.4.359-373.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crothers D. M., Zimm B. H. Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weight. J Mol Biol. 1965 Jul;12(3):525–536. doi: 10.1016/s0022-2836(65)80310-2. [DOI] [PubMed] [Google Scholar]
  6. DAEMS W. T. A preliminary report on the fine structure of a bacteriophage of Arthrobacter polychromogenes Schippers-Lammertse, Muysers et Klatser-Oedekerk. Antonie Van Leeuwenhoek. 1963;29:16–21. doi: 10.1007/BF02046034. [DOI] [PubMed] [Google Scholar]
  7. Freifelder D. Effect of NaCIO-4 on bacteriophage: release of DNA and evidence for population heterogeneity. Virology. 1966 Apr;28(4):742–750. doi: 10.1016/0042-6822(66)90258-3. [DOI] [PubMed] [Google Scholar]
  8. Jollick J. D. Differential phage sensitivity of cell types in Caulobacter. J Gen Virol. 1972 Sep;16(3):405–407. doi: 10.1099/0022-1317-16-3-405. [DOI] [PubMed] [Google Scholar]
  9. MARMUR J., FALKOW S., MANDEL M. NEW APPROACHES TO BACTERIAL TAXONOMY. Annu Rev Microbiol. 1963;17:329–372. doi: 10.1146/annurev.mi.17.100163.001553. [DOI] [PubMed] [Google Scholar]

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