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. 1973 Nov;12(5):1122–1130. doi: 10.1128/jvi.12.5.1122-1130.1973

Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron

Carlos B Fernandez-Tomas 1, David Baltimore 1
PMCID: PMC356744  PMID: 4358164

Abstract

Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125S, contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35S viral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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