Abstract
Replicative intermediates of adenovirus type 5 DNA contain large stretches of single-stranded DNA. We have shown that this single-stranded DNA is mainly of parental origin, whereas all new DNA synthesized during one round of replication has a double-stranded structure. Hybridization experiments of the single-stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the strand with the lower equilibrium density in alkaline CsCl) indicating that it represents the viral H-strand. This observation implies that replication always starts from one and the same molecular end. Electron microscopy of partially denatured Y-shaped intermediates confirmed this and showed that replication started from the molecular right end (the end richest in A-T base pairs). In conclusion, we have shown that replication of adenovirus type 5 DNA starts at the molecular right end, displacing the parental H-strand.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aloni Y., Winocour E., Sachs L., Torten J. Hybridization between SV40 DNA and cellular DNA's. J Mol Biol. 1969 Sep 14;44(2):333–345. doi: 10.1016/0022-2836(69)90179-x. [DOI] [PubMed] [Google Scholar]
- Bellett A. J., Younghusband H. B. Replication of the DNA of chick embryo lethal orphan virus. J Mol Biol. 1972 Dec 30;72(3):691–709. doi: 10.1016/0022-2836(72)90185-4. [DOI] [PubMed] [Google Scholar]
- Doerfler W., Kleinschmidt A. K. Denaturation pattern of the DNA of adenovirus type 2 as determined by electron microscopy. J Mol Biol. 1970 Jun 28;50(3):579–593. doi: 10.1016/0022-2836(70)90086-0. [DOI] [PubMed] [Google Scholar]
- Flavell R. A., Borst P., ter Schegget J. DNA synthesis by isolated mitochondria. IV. Isolation of an intermediate containing newly synthesized DNA in full-length light strands. Biochim Biophys Acta. 1972 Jul 20;272(3):341–349. [PubMed] [Google Scholar]
- Kiger J. A., Jr, Sinsheimer R. L. Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1969 Mar 28;40(3):467–490. doi: 10.1016/0022-2836(69)90166-1. [DOI] [PubMed] [Google Scholar]
- Sussenbach J. S., van der Vliet P. C., Ellens D. J., Jansz H. S. Linear intermediates in the replication of adenovirus DNA. Nat New Biol. 1972 Sep 13;239(89):47–49. [PubMed] [Google Scholar]
- Sussenbach J. S., van der Vliet P. C. Studies on the mechanism of replication of adenovirus DNA. I. The effect of hydroxyurea. Virology. 1973 Jul;54(1):299–303. doi: 10.1016/0042-6822(73)90142-6. [DOI] [PubMed] [Google Scholar]
- Sussenbach J. S., van der Vliet P. C. Viral DNA synthesis in isolated nuclei from adenovirus-infected KB cells. FEBS Lett. 1972 Mar;21(1):7–10. doi: 10.1016/0014-5793(72)80149-2. [DOI] [PubMed] [Google Scholar]
- van der Eb A. J. Intermediates in type 5 adenovirus DNA replication. Virology. 1973 Jan;51(1):11–23. doi: 10.1016/0042-6822(73)90361-9. [DOI] [PubMed] [Google Scholar]
- van der Vliet P. C., Sussenbach J. S. The mechanism of adenovirus-DNA synthesis in isolated nuclei. Eur J Biochem. 1972 Nov 7;30(3):584–592. doi: 10.1111/j.1432-1033.1972.tb02130.x. [DOI] [PubMed] [Google Scholar]