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. 1973 Nov;12(5):1149–1159. doi: 10.1128/jvi.12.5.1149-1159.1973

Viral Protein Synthesis in Bacteriophage φ29-Infected Bacillus subtilis

Louise A Hawley 1, Bernard E Reilly 1, Elaine W Hagen 1, Dwight L Anderson 1
PMCID: PMC356747  PMID: 4203085

Abstract

Twenty-three 14C-labeled phage φ29-specific proteins in lysates of UV-irradiated Bacillus subtilis have been resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and identified by autoradiography. Included in this group of proteins are the six major structural proteins of the virion. Analysis of the temporal sequence of viral protein synthesis indicates that three groups of proteins can be identified by time of appearance, beginning at 2 to 4, 4 to 6, or 8 to 10 min after infection, respectively. These proteins account for approximately 90% of the coding capacity of the φ29 genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. L., Hickman D. D., Reilly B. E. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid. J Bacteriol. 1966 May;91(5):2081–2089. doi: 10.1128/jb.91.5.2081-2089.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson D. L., Mosharrafa E. T. Physical and biological properties of phage phi 29 deoxyribonucleic acid. J Virol. 1968 Oct;2(10):1185–1190. doi: 10.1128/jvi.2.10.1185-1190.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  4. Dickson R. C., Barnes S. L., Eiserling F. A. Structural proteins of bacteriophage T4. J Mol Biol. 1970 Nov 14;53(3):461–474. doi: 10.1016/0022-2836(70)90077-x. [DOI] [PubMed] [Google Scholar]
  5. Hagen E. W., Zeece V. M., Anderson D. L. A genetic study of temperature-sensitive mutants of the Bacillus subtilis bacteriophage phi 29. Virology. 1971 Mar;43(3):561–568. doi: 10.1016/0042-6822(71)90281-9. [DOI] [PubMed] [Google Scholar]
  6. Hosoda J., Cone R. Analysis of T4 phage proteins. I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1275–1281. doi: 10.1073/pnas.66.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kellenberger E., Der Kamp C. K.-V. On a modification of the gene product P23 according to its use as subunit of either normal capsids of phage T4 or of polyheads. FEBS Lett. 1970 Jun 1;8(3):140–144. doi: 10.1016/0014-5793(70)80247-2. [DOI] [PubMed] [Google Scholar]
  8. Kellenberger E., Eiserling F. A., Boy de la Tour E. Studies on the morphopoiesis of the head of phage T-even. 3. The cores of head-related structures. J Ultrastruct Res. 1967 Dec 12;21(3):335–360. doi: 10.1016/s0022-5320(67)80099-6. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Loskutoff D. J., Pène J. J., Andrews D. P. Gene expression during the development of Bacillus subtilis bacteriophage phi 29. I. Analysis of viral-specific transcription by deoxyribonucleic acid-ribonucleic acid competition hybridization. J Virol. 1973 Jan;11(1):78–86. doi: 10.1128/jvi.11.1.78-86.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Loskutoff D. J., Pène J. J. Gene expression during the development of Bacillus subtilis bacteriophage phi29. II. Resolution of viral-specific ribonucleic acid molecules. J Virol. 1973 Jan;11(1):87–97. doi: 10.1128/jvi.11.1.87-97.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mosharrafa E. T., Schachtele C. F., Reilly B. E., Anderson D. L. Complementary Strands of Bacteriophage phi29 Deoxyribonucleic Acid: Preparative Separation and Transcription Studies. J Virol. 1970 Dec;6(6):855–864. doi: 10.1128/jvi.6.6.855-864.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Méndez E., Ramírez G., Salas M., Viñuela E. Structural proteins of bacteriophage phi 29. Virology. 1971 Sep;45(3):567–576. doi: 10.1016/0042-6822(71)90172-3. [DOI] [PubMed] [Google Scholar]
  14. Ortin J., Viñuela E., Salas M., Vasquez C. DNA-protein complex in circular DNA from phage phi-29. Nat New Biol. 1971 Dec 29;234(52):275–277. doi: 10.1038/newbio234275a0. [DOI] [PubMed] [Google Scholar]
  15. Polsinelli M., Beretta M. Genetic Recombination in Crosses Between Streptomyces aureofaciens and Streptomyces rimosus. J Bacteriol. 1966 Jan;91(1):63–68. doi: 10.1128/jb.91.1.63-68.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. REILLY B. E., SPIZIZEN J. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS. J Bacteriol. 1965 Mar;89:782–790. doi: 10.1128/jb.89.3.782-790.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reilly B. E., Zeece V. M., Anderson D. L. Genetic study of suppressor-sensitive mutants of the Bacillus subtilis bacteriophage phi 29. J Virol. 1973 May;11(5):756–760. doi: 10.1128/jvi.11.5.756-760.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schachtele C. F., De Sain C. V., Anderson D. L. Transcription during the development of bacteriophage phi29: definition of "early" and "late" phi29 ribonucleic acid. J Virol. 1973 Jan;11(1):9–16. doi: 10.1128/jvi.11.1.9-16.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schachtele C. F., De Sain C. V., Hawley L. A., Anderson D. L. Transcription during the development of bacteriophage phi 29: production of host- and phi 29-specific ribonucleic acid. J Virol. 1972 Dec;10(6):1170–1178. doi: 10.1128/jvi.10.6.1170-1178.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schachtele C. F., Reilly B. E., De Sain C. V., Whittington M. O., Anderson D. L. Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis. J Virol. 1973 Jan;11(1):153–155. doi: 10.1128/jvi.11.1.153-155.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]

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