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. 1973 Nov;12(5):1164–1172. doi: 10.1128/jvi.12.5.1164-1172.1973

Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells

Evelyn Albu a,1, Kathryn V Holmes a,2
PMCID: PMC356749  PMID: 4797266

Abstract

An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70S in sucrose density gradients and has a molecular weight of approximately 107, as estimated by gel electrophoresis. The R-type VLP can be labeled with 3H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.

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