Abstract
Cytological preparations of cells transformed by members of three groups of human adenoviruses, adenovirus 12, 7, and 2, were annealed with radioactive complementary RNA (cRNA) (4 × 107 to 4.5 × 107 dpm/μg) prepared by copying viral DNA with the Escherichia coli DNA-directed RNA polymerase. These in situ hybridizations detected adenovirus-specific DNA sequences in interphase nuclei when transformed cells were annealed with homologous viral cRNA, but not with heterologous viral cRNA. The highest autoradiographic grain counts were found over adenovirus 7-transformed cell nuclei, next over adenovirus 12-, and the lowest over adenovirus 2-transformed cell nuclei. This is the same order as found by reassociation kinetic measurements (K. Fujinaga and M. Green, unpublished data).
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- Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
- Bishop J. O., Jones K. W. Chromosomal localization of human haemoglobin structural genes. Nature. 1972 Nov 17;240(5377):149–150. doi: 10.1038/240149a0. [DOI] [PubMed] [Google Scholar]
- Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
- GREEN M. Biochemical studies on adenovirus multiplication. 1. Stimulation of phosphorus incorporation into deoxyribonucleic acid and ribouncleic acid. Virology. 1959 Nov;9:343–358. doi: 10.1016/0042-6822(59)90127-8. [DOI] [PubMed] [Google Scholar]
- GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gall J. G., Pardue M. L. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A. 1969 Jun;63(2):378–383. doi: 10.1073/pnas.63.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green M., Hodap M. Asymmetric transcription of early adenovirus genes by the Escherichia coli RNA polymerase in vitro. J Mol Biol. 1972 Feb 28;64(1):305–309. doi: 10.1016/0022-2836(72)90338-5. [DOI] [PubMed] [Google Scholar]
- Green M. Molecular basis for the attack on cancer. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1036–1041. doi: 10.1073/pnas.69.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones K. W. Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature. 1970 Mar 7;225(5236):912–915. doi: 10.1038/225912a0. [DOI] [PubMed] [Google Scholar]
- McDougall J. K., Dunn A. R., Jones K. W. In situ hybridization of adenovirus RNA and DNA. Nature. 1972 Apr 14;236(5346):346–348. doi: 10.1038/236346a0. [DOI] [PubMed] [Google Scholar]
- Orth G., Jeanteur P., Croissant O. Evidence for and localization of vegetative viral DNA replication by autoradiographic detection of RNA-DNA hybrids in sections of tumors induced by Shope papilloma virus. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1876–1880. doi: 10.1073/pnas.68.8.1876. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pettersson U., Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. doi: 10.1016/0022-2836(73)90164-2. [DOI] [PubMed] [Google Scholar]