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. 1973 Dec;12(6):1325–1335. doi: 10.1128/jvi.12.6.1325-1335.1973

L Protein Requirement for In Vitro RNA Synthesis by Vesicular Stomatitis Virus

Suzanne U Emerson 1, Robert R Wagner 1
PMCID: PMC356774  PMID: 4357510

Abstract

The endogenous transcriptase present in purified vesicular stomatitis (VS) virions was solubilized with a Triton X-100 high-salt solution. The polymerase activity was purified on glycerol gradients and by phosphocellulose column chromatography; the viral proteins present in the active enzyme fractions were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis. It was demonstrated that L protein, but not NS protein, was required for in vitro RNA synthesis on the VS viral nucleocapsid template. Solubilized L protein rebinds to the ribonucleoprotein template when the transcription complex is reconstituted, and the RNA synthesized in vitro by purified L protein hybridizes to virion RNA. Cyanogen bromide peptide fingerprints indicate that the large L protein is a unique polypeptide chain. It is concluded that the L protein functions as the transcriptase, and the nucleocapsid NS protein is not essential for in vitro RNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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