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. 1973 Dec;12(6):1427–1433. doi: 10.1128/jvi.12.6.1427-1433.1973

Studies on the Biosynthesis of α-Putrescinylthymine in Bacteriophage φW-14-Infected Pseudomonas acidovorans

Rod A Kelln a,1, R A J Warren a
PMCID: PMC356784  PMID: 4586777

Abstract

The α-putrescinylthymine (putThy) in bacteriophage φW-14 DNA is synthesized at the mononucleotide level: it is labeled by uracil or deoxyuridine but not by thymidine, and it appears in the acid-soluble pool of infected cells before the onset of phage DNA synthesis. The methylene group at the C-5 position of the pyrimidine moiety of putThy is derived in vivo from a C1 unit. Extracts of a phage infected thymidine auxotroph of the host, Pseudomonas acidovorans, apparently contain a phage-specific thymidylate synthetase and a phage-specific activity which forms 5-hydroxymethyl dUMP from N5, N10-methylene-tetrahydrofolate and dUMP.

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Selected References

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