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. 1973 Dec;12(6):1466–1472. doi: 10.1128/jvi.12.6.1466-1472.1973

Synthesis of Bacteriophage M13-Specific Proteins in a DNA-Dependent Cell-Free System II. In Vitro Synthesis of Biologically Active Gene 5 Protein

Ruud N H Konings 1, Josephine Jansen 1, Theo Cuypers 1, John G G Schoenmakers 1
PMCID: PMC356789  PMID: 4586780

Abstract

It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. Like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, DNAs. These results suggest that truly functional gene 5 protein is made in the cell-free system.

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Selected References

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