Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1973 Dec;12(6):1534–1539. doi: 10.1128/jvi.12.6.1534-1539.1973

Morphological Variants of Sindbis Virus Obtained from Infected Mosquito Tissue Culture Cells

Dennis T Brown a, Jeffrey B Gliedman a,1
PMCID: PMC356796  PMID: 4128381

Abstract

Tissue-cultured Aedes albopictus cells infected with morphologically homogeneous Sindbis virus were found to produce progeny virions which could be divided into three classes based on size. The thickness of the envelope was constant on all three sizes of progeny virions suggesting that the variability in size rested with the viral nucleocapsid. It is suggested that the three classes of virions have icosahedral nucleocapsids composed of common subunits organized in decreasing triangulation numbers.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson N. H., Tamm I. Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967 May;32(1):128–143. doi: 10.1016/0042-6822(67)90261-9. [DOI] [PubMed] [Google Scholar]
  2. Bayer M. E., Remsen C. C. Structure of Escherichia coli after freeze-etching. J Bacteriol. 1970 Jan;101(1):304–313. doi: 10.1128/jb.101.1.304-313.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown D. T., Waite M. R., Pfefferkorn E. R. Morphology and morphogenesis of Sindbis virus as seen with freeze-etching techniques. J Virol. 1972 Sep;10(3):524–536. doi: 10.1128/jvi.10.3.524-536.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CASPAR D. L., KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. doi: 10.1101/sqb.1962.027.001.005. [DOI] [PubMed] [Google Scholar]
  5. Compans R. W. Location of the glycoprotein in the membrane of Sindbis virus. Nat New Biol. 1971 Jan 27;229(4):114–116. doi: 10.1038/newbio229114a0. [DOI] [PubMed] [Google Scholar]
  6. Grimley P. M., Berezesky I. K., Friedman R. M. Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol. 1968 Nov;2(11):1326–1338. doi: 10.1128/jvi.2.11.1326-1338.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Horzinek M., Mussgay M. Studies on the nucleocapsid structure of a group A arbovirus. J Virol. 1969 Oct;4(4):514–520. doi: 10.1128/jvi.4.4.514-520.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PFEFFERKORN E. R., CLIFFORD R. L. THE ORIGIN OF THE PROTEIN OF SINDBIS VIRUS. Virology. 1964 Jun;23:217–223. doi: 10.1016/0042-6822(64)90285-5. [DOI] [PubMed] [Google Scholar]
  10. Schlesinger S., Schlesinger M., Burge B. W. Defective virus particles from Sindbis virus. Virology. 1972 May;48(2):615–617. doi: 10.1016/0042-6822(72)90076-1. [DOI] [PubMed] [Google Scholar]
  11. Shenk T. E., Stollar V. Viral RNA species in BHK-21 cells infected with Sindbis virus serially passaged at high multiplicity of infection. Biochem Biophys Res Commun. 1972 Oct 6;49(1):60–67. doi: 10.1016/0006-291x(72)90009-5. [DOI] [PubMed] [Google Scholar]
  12. Simpson R. W., Hauser R. E. Basic structure of group A arbovirus strains Middelburg, Sindbis, and Semliki Forest examined by negative staining. Virology. 1968 Feb;34(2):358–361. doi: 10.1016/0042-6822(68)90248-1. [DOI] [PubMed] [Google Scholar]
  13. Stevens T. M. Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture. Proc Soc Exp Biol Med. 1970 May;134(1):356–361. doi: 10.3181/00379727-134-34793. [DOI] [PubMed] [Google Scholar]
  14. Stollar V., Shenk T. E. Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. J Virol. 1973 Apr;11(4):592–595. doi: 10.1128/jvi.11.4.592-595.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES