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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Methods. 2012 Jun 13;59(2):225–232. doi: 10.1016/j.ymeth.2012.05.011

Figure 4.

Figure 4

A. Native PAGE followed by immunoblot analysis for IRF-3 in SupT1 cells infected with SenV for 18 hrs. A rabbit polyclonal antibody or AR-1 mAb was used to detect IRF-3. Arrows indicate monomeric IRF-3. Short (top) and long (bottom) film exposures are shown.

B. Native PAGE followed by immunoblot analysis for IRF-3 using supernatants from five hybridoma clones. Clone number 1 represents AR-1 mAb, while clone number 2 represents AR-2 mAb. Short and long film exposures of each immunoblot assay are shown as in 4A.

C. SDS-PAGE followed by immunoblot analysis for IRF-3 (AR-1) for loading control.