Table 2.
Summary of antibodies used in the immunohistochemical analysis of mTOR pathway.
Pten: phosphatase and tensine homologue; p27: p27kip; phos Akt: phoporylated Akt; phos S6: phosphorylated S6 protein; 4E-BP1: eukaryotic translation initiation factor 4E-binding protein-1.
| Vendor | Clone | Pre-treatment | Dilution | Incubation | Detection | |
|---|---|---|---|---|---|---|
| Pten | Cell Signaling | D4.3 | EDTA 45 min | 1:50 | overnight 4C | PowerVision |
| phos Akt | Cell Signaling | 736E11 | EDTA 45 min | 1:50 | 45 min room T | PowerVision |
| phos S6 | Cell Signaling | polyclonal | EDTA 45 min | 1:200 | overnight 4C | Envision |
| 4E-BP1 | ProSci | polyclonal | citrate 25 min | 1:250 | 45 min room T | Envision |
| c-Myc | Epitomics | Y69 | EDTA 45 min | 1:300 | overnight 4C | CSA kit |
| p27 | Transduction lab | 57 | citrate 25 min | 1:4k | 45 min room T | PowerVision |