Skip to main content
. Author manuscript; available in PMC: 2013 Feb 11.
Published in final edited form as: Transfusion. 2009 Nov 9;50(3):642–648. doi: 10.1111/j.1537-2995.2009.02481.x

Fig. 1.

Fig. 1

HOD RBCs stored for 14 days result in higher levels of alloantibodies after transfusion. HOD RBCs were filter leukoreduced and transfused fresh or stored for 14 days and then transfused. Alloantibody response was assessed 14 days after transfusion. (A) Anti-HEL response as determined by ELISA in a representative experiment with five mice per group, transfused with fresh leukoreduced HOD RBCs (–●–) or 14-day-stored leukoreduced HOD RBCs (--▲--); mean and SD are shown with sera dilutions of 1:50–1:500,000. This experiment was repeated five times (five to six mice/group/experiment) with similar results. (B) Anti-HOD response as determined by flow cytometric crossmatch. Representative flow histograms are shown, with serum at a 1:5 dilution from a recipient of fresh HOD RBCs and from a recipient of 14-day-stored HOD RBCs crossmatched with control FVB RBCs (shaded) or HOD RBCs (solid).