Figure 3.

Addition of freshly activated encephalitogenic cells exacerbates chronic classical experimental autoimmune encephalomyelitis (cEAE) disease regardless of the capacity of new cells to produce interferon‐γ (IFN‐γ). Mice received 5 × 10⁶ wild‐type MOG_35–55‐specific CD4⁺ T cells (where MOG_35–55 is a peptide comprising amino acids 35 to 55 of the myelin oligodendrocyte glycoprotein). After 23 days (a) or 90 days (b) mice received 5 × 10⁶ of an IFN‐γ‐deficient MOG_35–55‐specific cell line (●), an IFN‐γ‐sufficient ovalbumin_‐specific cell line (▲), or the initially injected IFN‐γ‐sufficient MOG_35–55‐specific T‐cell line (■). Mice were then followed for the clinical symptoms of cEAE over time. Results shown were collected from eight mice (a) or 10–12 mice (b) in each group examined over two experiments.