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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Microcirculation. 2013 Feb;20(2):170–182. doi: 10.1111/micc.12016

Figure 6. Effect of chronic occlusion and exercise training on superoxide dismutase protein and activity levels in coronary arterioles.

Figure 6

Immunoblot analyses of the superoxide dismutase (SOD) family of isoforms revealed that neither ecSOD (A), MnSOD (B), nor Cu/ZnSOD (C) protein levels were significantly altered by occlusion or exercise training. Protein was quantified by densitometry analysis, normalized to β-actin, and expressed relative to the density of nonoccluded arterioles of sedentary (SED) pigs. Evaluation of SOD activity also demonstrated no significant different between arteriolar treatment groups. Furthermore, bradykinin (BK; 10 nM) treatment had no effect on SOD activity compared with control (PSS; conditions). EX, exercise-trained. Values are means ± S.E.M. of the number of animals indicated.