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. 1991 Apr;4(2):156–168. doi: 10.1128/cmr.4.2.156

Nucleic acid detection systems for enteroviruses.

H A Rotbart 1
PMCID: PMC358188  PMID: 1649002

Abstract

The enteroviruses comprise nearly 70 human pathogens responsible for a wide array of diseases including poliomyelitis, meningitis, myocarditis, and neonatal sepsis. Current diagnostic tests for the enteroviruses are limited in their use by the slow growth, or failure to grow, of certain serotypes in culture, the antigenic diversity among the serotypes, and the low titer of virus in certain clinical specimens. Within the past 6 years, applications of molecular cloning techniques, in vitro transcription vectors, automated nucleic acid synthesis, and the polymerase chain reaction have resulted in significant progress toward nucleic acid-based detection systems for the enteroviruses that take advantage of conserved genomic sequences across many, if not all, serotypes. Similar approaches to the study of enteroviral pathogenesis have already produced dramatic advances in our understanding of how these important viruses cause their diverse clinical spectra.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abzug M. J., Rotbart H. A., Levin M. J. Demonstration of a barrier to transplacental passage of murine enteroviruses in late gestation. J Infect Dis. 1989 Apr;159(4):761–765. doi: 10.1093/infdis/159.4.761. [DOI] [PubMed] [Google Scholar]
  2. Alksnis M., Lindberg M., Stålhandske P., Hultberg H., Pettersson U. Use of synthetic oligodeoxyribonucleotides for type-specific identification of coxsackie B viruses. Mol Cell Probes. 1989 Jun;3(2):103–108. doi: 10.1016/0890-8508(89)90020-0. [DOI] [PubMed] [Google Scholar]
  3. Archard L. C., Bowles N. E., Behan P. O., Bell E. J., Doyle D. Postviral fatigue syndrome: persistence of enterovirus RNA in muscle and elevated creatine kinase. J R Soc Med. 1988 Jun;81(6):326–329. doi: 10.1177/014107688808100608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Assaad F., Ljungars-Esteves K. World overview of poliomyelitis: regional patterns and trends. Rev Infect Dis. 1984 May-Jun;6 (Suppl 2):S302–S307. doi: 10.1093/clinids/6.supplement_2.s302. [DOI] [PubMed] [Google Scholar]
  5. BODIAN D. Emerging concept of poliomyelitis infection. Science. 1955 Jul 15;122(3159):105–108. doi: 10.1126/science.122.3159.105. [DOI] [PubMed] [Google Scholar]
  6. Baltimore D., Girard M. An intermediate in the synthesis of poliovirus RNA. Proc Natl Acad Sci U S A. 1966 Aug;56(2):741–748. doi: 10.1073/pnas.56.2.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Barrett-Connor E. Is insulin-dependent diabetes mellitus caused by coxsackievirus B infection? A review of the epidemiologic evidence. Rev Infect Dis. 1985 Mar-Apr;7(2):207–215. doi: 10.1093/clinids/7.2.207. [DOI] [PubMed] [Google Scholar]
  8. Bell E. J., McCartney R. A., Basquill D., Chaudhuri A. K. Mu-antibody capture ELISA for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis. J Med Virol. 1986 Jul;19(3):213–217. doi: 10.1002/jmv.1890190303. [DOI] [PubMed] [Google Scholar]
  9. Bowles N. E., Dubowitz V., Sewry C. A., Archard L. C. Dermatomyositis, polymyositis, and Coxsackie-B-virus infection. Lancet. 1987 May 2;1(8540):1004–1007. doi: 10.1016/s0140-6736(87)92271-9. [DOI] [PubMed] [Google Scholar]
  10. Bowles N. E., Richardson P. J., Olsen E. G., Archard L. C. Detection of Coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. Lancet. 1986 May 17;1(8490):1120–1123. doi: 10.1016/s0140-6736(86)91837-4. [DOI] [PubMed] [Google Scholar]
  11. Brahic M., Haase A. T., Cash E. Simultaneous in situ detection of viral RNA and antigens. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5445–5448. doi: 10.1073/pnas.81.17.5445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Brahic M., Stroop W. G., Baringer J. R. Theiler's virus persists in glial cells during demyelinating disease. Cell. 1981 Oct;26(1 Pt 1):123–128. doi: 10.1016/0092-8674(81)90040-4. [DOI] [PubMed] [Google Scholar]
  13. Bruce C., al-Nakib W., Forsyth M., Stanway G., Almond J. W. Detection of enteroviruses using cDNA and synthetic oligonucleotide probes. J Virol Methods. 1989 Aug;25(2):233–240. doi: 10.1016/0166-0934(89)90035-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cash E., Chamorro M., Brahic M. Minus-strand RNA synthesis in the spinal cords of mice persistently infected with Theiler's virus. J Virol. 1988 May;62(5):1824–1826. doi: 10.1128/jvi.62.5.1824-1826.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Chang K. H., Auvinen P., Hyypiä T., Stanway G. The nucleotide sequence of coxsackievirus A9; implications for receptor binding and enterovirus classification. J Gen Virol. 1989 Dec;70(Pt 12):3269–3280. doi: 10.1099/0022-1317-70-12-3269. [DOI] [PubMed] [Google Scholar]
  16. Chapman N. M., Tracy S., Gauntt C. J., Fortmueller U. Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization. J Clin Microbiol. 1990 May;28(5):843–850. doi: 10.1128/jcm.28.5.843-850.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Chonmaitree T., Ford C., Sanders C., Lucia H. L. Comparison of cell cultures for rapid isolation of enteroviruses. J Clin Microbiol. 1988 Dec;26(12):2576–2580. doi: 10.1128/jcm.26.12.2576-2580.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Chonmaitree T., Menegus M. A., Powell K. R. The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY. JAMA. 1982 Apr 2;247(13):1843–1847. [PubMed] [Google Scholar]
  19. Cohen J. I., Ticehurst J. R., Purcell R. H., Buckler-White A., Baroudy B. M. Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses. J Virol. 1987 Jan;61(1):50–59. doi: 10.1128/jvi.61.1.50-59.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Coller B. A., Chapman N. M., Beck M. A., Pallansch M. A., Gauntt C. J., Tracy S. M. Echovirus 22 is an atypical enterovirus. J Virol. 1990 Jun;64(6):2692–2701. doi: 10.1128/jvi.64.6.2692-2701.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Couderc T., Christodoulou C., Kopecka H., Marsden S., Taffs L. F., Crainic R., Horaud F. Molecular pathogenesis of neural lesions induced by poliovirus type 1. J Gen Virol. 1989 Nov;70(Pt 11):2907–2918. doi: 10.1099/0022-1317-70-11-2907. [DOI] [PubMed] [Google Scholar]
  22. Cova L., Kopecka H., Aymard M., Girard M. Use of cRNA probes for the detection of enteroviruses by molecular hybridization. J Med Virol. 1988 Jan;24(1):11–18. doi: 10.1002/jmv.1890240103. [DOI] [PubMed] [Google Scholar]
  23. Cronin M. E., Love L. A., Miller F. W., McClintock P. R., Plotz P. H. The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization. J Exp Med. 1988 Nov 1;168(5):1639–1648. doi: 10.1084/jem.168.5.1639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Cumakov I. M., Lipskaya G. Y., Agol V. I. Comparative studies on the genomes of some picornaviruses: denaturation mapping of replicative form RNA and electron microscopy of heteroduplex RNA. Virology. 1979 Jan 30;92(2):259–270. doi: 10.1016/0042-6822(79)90130-2. [DOI] [PubMed] [Google Scholar]
  25. Dagan R., Menegus M. A. A combination of four cell types for rapid detection of enteroviruses in clinical specimens. J Med Virol. 1986 Jul;19(3):219–228. doi: 10.1002/jmv.1890190304. [DOI] [PubMed] [Google Scholar]
  26. Dalakas M. C., Sever J. L., Madden D. L., Papadopoulos N. M., Shekarchi I. C., Albrecht P., Krezlewicz A. Late postpoliomyelitis muscular atrophy: clinical, virologic, and immunologic studies. Rev Infect Dis. 1984 May-Jun;6 (Suppl 2):S562–S567. doi: 10.1093/clinids/6.supplement_2.s562. [DOI] [PubMed] [Google Scholar]
  27. Dalldorf G., Sickles G. M. An Unidentified, Filtrable Agent Isolated From the Feces of Children With Paralysis. Science. 1948 Jul 16;108(2794):61–62. doi: 10.1126/science.108.2794.61. [DOI] [PubMed] [Google Scholar]
  28. Dörries R., ter Meulen V. Specificity of IgM antibodies in acute human coxsackievirus B infections, analysed by indirect solid phase enzyme immunoassay and immunoblot technique. J Gen Virol. 1983 Jan;64(Pt 1):159–167. doi: 10.1099/0022-1317-64-1-159. [DOI] [PubMed] [Google Scholar]
  29. Easton A. J., Eglin R. P. The detection of coxsackievirus RNA in cardiac tissue by in situ hybridization. J Gen Virol. 1988 Feb;69(Pt 2):285–291. doi: 10.1099/0022-1317-69-2-285. [DOI] [PubMed] [Google Scholar]
  30. Enders J. F., Weller T. H., Robbins F. C. Cultivation of the Lansing Strain of Poliomyelitis Virus in Cultures of Various Human Embryonic Tissues. Science. 1949 Jan 28;109(2822):85–87. doi: 10.1126/science.109.2822.85. [DOI] [PubMed] [Google Scholar]
  31. Fallis R. J., Weiner L. P. Further studies in search of a virus in amyotrophic lateral sclerosis. Adv Neurol. 1982;36:355–361. [PubMed] [Google Scholar]
  32. Gauntt C. J., Gudvangen R. J., Brans Y. W., Marlin A. E. Coxsackievirus group B antibodies in the ventricular fluid of infants with severe anatomic defects in the central nervous system. Pediatrics. 1985 Jul;76(1):64–68. [PubMed] [Google Scholar]
  33. Green M. R., Maniatis T., Melton D. A. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell. 1983 Mar;32(3):681–694. doi: 10.1016/0092-8674(83)90054-5. [DOI] [PubMed] [Google Scholar]
  34. Grist N. R., Bell E. J. Paralytic poliomyelitis and nonpolio enteroviruses: studies in Scotland. Rev Infect Dis. 1984 May-Jun;6 (Suppl 2):S385–S386. doi: 10.1093/clinids/6.supplement_2.s385. [DOI] [PubMed] [Google Scholar]
  35. Hallam N. F., Eglin R. P., Holland P., Bell E. J., Squier M. V. Fatal Coxsackie B meningoencephalitis diagnosed by serology and in-situ nucleic acid hybridisation. Lancet. 1986 Nov 22;2(8517):1213–1214. doi: 10.1016/s0140-6736(86)92216-6. [DOI] [PubMed] [Google Scholar]
  36. Herrmann J. E., Hendry R. M., Collins M. F. Factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. J Clin Microbiol. 1979 Aug;10(2):210–217. doi: 10.1128/jcm.10.2.210-217.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Hewlett M. J., Florkiewicz R. Z. Sequence of picornavirus RNAs containing a radioiodinated 5'-linked peptide reveals a conserved 5' sequence. Proc Natl Acad Sci U S A. 1980 Jan;77(1):303–307. doi: 10.1073/pnas.77.1.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Hogle J. M., Chow M., Filman D. J. Three-dimensional structure of poliovirus at 2.9 A resolution. Science. 1985 Sep 27;229(4720):1358–1365. doi: 10.1126/science.2994218. [DOI] [PubMed] [Google Scholar]
  39. Hughes P. J., North C., Minor P. D., Stanway G. The complete nucleotide sequence of coxsackievirus A21. J Gen Virol. 1989 Nov;70(Pt 11):2943–2952. doi: 10.1099/0022-1317-70-11-2943. [DOI] [PubMed] [Google Scholar]
  40. Hyypiä T., Auvinen P., Maaronen M. Polymerase chain reaction for human picornaviruses. J Gen Virol. 1989 Dec;70(Pt 12):3261–3268. doi: 10.1099/0022-1317-70-12-3261. [DOI] [PubMed] [Google Scholar]
  41. Hyypiä T., Maaronen M., Auvinen P., Stålhandske P., Pettersson U., Stanway G., Hughes P., Ryan M., Almond J., Stenvik M. Nucleic acid sequence relationships between enterovirus serotypes. Mol Cell Probes. 1987 Jun;1(2):169–176. doi: 10.1016/0890-8508(87)90025-9. [DOI] [PubMed] [Google Scholar]
  42. Hyypiä T., Stålhandske P., Vainionpä R., Pettersson U. Detection of enteroviruses by spot hybridization. J Clin Microbiol. 1984 Mar;19(3):436–438. doi: 10.1128/jcm.19.3.436-438.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Iizuka N., Kuge S., Nomoto A. Complete nucleotide sequence of the genome of coxsackievirus B1. Virology. 1987 Jan;156(1):64–73. doi: 10.1016/0042-6822(87)90436-3. [DOI] [PubMed] [Google Scholar]
  44. Jarvis W. R., Tucker G. Echovirus type 7 meningitis in young children. Am J Dis Child. 1981 Nov;135(11):1009–1012. doi: 10.1001/archpedi.1981.02130350013006. [DOI] [PubMed] [Google Scholar]
  45. Jenkins O., Booth J. D., Minor P. D., Almond J. W. The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the Picornaviridae. J Gen Virol. 1987 Jul;68(Pt 7):1835–1848. doi: 10.1099/0022-1317-68-7-1835. [DOI] [PubMed] [Google Scholar]
  46. Johnson R. T. Late progression of poliomyelitis paralysis: discussion of pathogenesis. Rev Infect Dis. 1984 May-Jun;6 (Suppl 2):S568–S570. doi: 10.1093/clinids/6.supplement_2.s568. [DOI] [PubMed] [Google Scholar]
  47. Jubelt B., Wilson A. K., Ropka S. L., Guidinger P. L., McKinlay M. A. Clearance of a persistent human enterovirus infection of the mouse central nervous system by the antiviral agent disoxaril. J Infect Dis. 1989 May;159(5):866–871. doi: 10.1093/infdis/159.5.866. [DOI] [PubMed] [Google Scholar]
  48. Kandolf R., Ameis D., Kirschner P., Canu A., Hofschneider P. H. In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6272–6276. doi: 10.1073/pnas.84.17.6272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Kaplan M. H., Klein S. W., McPhee J., Harper R. G. Group B coxsackievirus infections in infants younger than three months of age: a serious childhood illness. Rev Infect Dis. 1983 Nov-Dec;5(6):1019–1032. doi: 10.1093/clinids/5.6.1019. [DOI] [PubMed] [Google Scholar]
  50. Leslie K., Blay R., Haisch C., Lodge A., Weller A., Huber S. Clinical and experimental aspects of viral myocarditis. Clin Microbiol Rev. 1989 Apr;2(2):191–203. doi: 10.1128/cmr.2.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Lindberg A. M., Stålhandske P. O., Pettersson U. Genome of coxsackievirus B3. Virology. 1987 Jan;156(1):50–63. doi: 10.1016/0042-6822(87)90435-1. [DOI] [PubMed] [Google Scholar]
  52. Lipson S. M., Walderman R., Costello P., Szabo K. Sensitivity of rhabdomyosarcoma and guinea pig embryo cell cultures to field isolates of difficult-to-cultivate group A coxsackieviruses. J Clin Microbiol. 1988 Jul;26(7):1298–1303. doi: 10.1128/jcm.26.7.1298-1303.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Lipton H. L., Kratochvil J., Sethi P., Dal Canto M. C. Theiler's virus antigen detected in mouse spinal cord 2 1/2 years after infection. Neurology. 1984 Aug;34(8):1117–1119. doi: 10.1212/wnl.34.8.1117. [DOI] [PubMed] [Google Scholar]
  54. Lipton H. L. Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination. Infect Immun. 1975 May;11(5):1147–1155. doi: 10.1128/iai.11.5.1147-1155.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. McCartney R. A., Banatvala J. E., Bell E. J. Routine use of mu-antibody-capture ELISA for the serological diagnosis of Coxsackie B virus infections. J Med Virol. 1986 Jul;19(3):205–212. doi: 10.1002/jmv.1890190302. [DOI] [PubMed] [Google Scholar]
  56. McKinlay M. A., Frank J. A., Jr, Benziger D. P., Steinberg B. A. Use of WIN 51711 to prevent echovirus type 9-induced paralysis in suckling mice. J Infect Dis. 1986 Oct;154(4):676–681. doi: 10.1093/infdis/154.4.676. [DOI] [PubMed] [Google Scholar]
  57. McKinney R. E., Jr, Katz S. L., Wilfert C. M. Chronic enteroviral meningoencephalitis in agammaglobulinemic patients. Rev Infect Dis. 1987 Mar-Apr;9(2):334–356. doi: 10.1093/clinids/9.2.334. [DOI] [PubMed] [Google Scholar]
  58. McSharry J. J., Caliguiri L. A., Eggers H. J. Inhibition of uncoating of poliovirus by arildone, a new antiviral drug. Virology. 1979 Sep;97(2):307–315. doi: 10.1016/0042-6822(79)90342-8. [DOI] [PubMed] [Google Scholar]
  59. Melnick J. L. Enterovirus type 71 infections: a varied clinical pattern sometimes mimicking paralytic poliomyelitis. Rev Infect Dis. 1984 May-Jun;6 (Suppl 2):S387–S390. doi: 10.1093/clinids/6.supplement_2.s387. [DOI] [PubMed] [Google Scholar]
  60. Mirkovic R. R., Kono R., Yin-Murphy M., Sohier R., Schmidt N. J., Melnick J. L. Enterovirus type 70: the etiologic agent of pandemic acute haemorrhagic conjunctivitis. Bull World Health Organ. 1973;49(4):341–346. [PMC free article] [PubMed] [Google Scholar]
  61. Modlin J. F. Perinatal echovirus infection: insights from a literature review of 61 cases of serious infection and 16 outbreaks in nurseries. Rev Infect Dis. 1986 Nov-Dec;8(6):918–926. doi: 10.1093/clinids/8.6.918. [DOI] [PubMed] [Google Scholar]
  62. Moore M. Centers for Disease Control. Enteroviral disease in the United States, 1970-1979. J Infect Dis. 1982 Jul;146(1):103–108. doi: 10.1093/infdis/146.1.103. [DOI] [PubMed] [Google Scholar]
  63. Morens D. M. Enteroviral disease in early infancy. J Pediatr. 1978 Mar;92(3):374–377. doi: 10.1016/s0022-3476(78)80422-3. [DOI] [PubMed] [Google Scholar]
  64. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  65. Newman C. L., Modlin J., Yolken R. H., Viscidi R. P. Solution hybridization and enzyme immunoassay for biotinylated DNA-RNA hybrids to detect enteroviral RNA in cell culture. Mol Cell Probes. 1989 Dec;3(4):375–382. doi: 10.1016/0890-8508(89)90016-9. [DOI] [PubMed] [Google Scholar]
  66. Otto M. J., Fox M. P., Fancher M. J., Kuhrt M. F., Diana G. D., McKinlay M. A. In vitro activity of WIN 51711, a new broad-spectrum antipicornavirus drug. Antimicrob Agents Chemother. 1985 Jun;27(6):883–886. doi: 10.1128/aac.27.6.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Petitjean J., Quibriac M., Freymuth F., Fuchs F., Laconche N., Aymard M., Kopecka H. Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. J Clin Microbiol. 1990 Feb;28(2):307–311. doi: 10.1128/jcm.28.2.307-311.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Riedel B. D., Ghishan F. K. Maturation of chloride-bicarbonate exchange in rat ileal brush border membrane vesicles. Pediatr Res. 1989 Feb;25(2):189–193. doi: 10.1203/00006450-198902000-00022. [DOI] [PubMed] [Google Scholar]
  69. Rosenberg N. L., Rotbart H. A., Abzug M. J., Ringel S. P., Levin M. J. Evidence for a novel picornavirus in human dermatomyositis. Ann Neurol. 1989 Aug;26(2):204–209. doi: 10.1002/ana.410260204. [DOI] [PubMed] [Google Scholar]
  70. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature. 1985 Sep 12;317(6033):145–153. doi: 10.1038/317145a0. [DOI] [PubMed] [Google Scholar]
  71. Rotbart H. A., Abzug M. J., Levin M. J. Development and application of RNA probes for the study of picornaviruses. Mol Cell Probes. 1988 Mar;2(1):65–73. doi: 10.1016/0890-8508(88)90045-x. [DOI] [PubMed] [Google Scholar]
  72. Rotbart H. A., Abzug M. J., Murray R. S., Murphy N. L., Levin M. J. Intracellular detection of sense and antisense enteroviral RNA by in situ hybridization. J Virol Methods. 1988 Dec;22(2-3):295–301. doi: 10.1016/0166-0934(88)90111-5. [DOI] [PubMed] [Google Scholar]
  73. Rotbart H. A. Diagnosis of enteroviral meningitis with the polymerase chain reaction. J Pediatr. 1990 Jul;117(1 Pt 1):85–89. doi: 10.1016/s0022-3476(05)82451-5. [DOI] [PubMed] [Google Scholar]
  74. Rotbart H. A., Eastman P. S., Ruth J. L., Hirata K. K., Levin M. J. Nonisotopic oligomeric probes for the human enteroviruses. J Clin Microbiol. 1988 Dec;26(12):2669–2671. doi: 10.1128/jcm.26.12.2669-2671.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Rotbart H. A. Enzymatic RNA amplification of the enteroviruses. J Clin Microbiol. 1990 Mar;28(3):438–442. doi: 10.1128/jcm.28.3.438-442.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Rotbart H. A., Kinsella J. P., Wasserman R. L. Persistent enterovirus infection in culture-negative meningoencephalitis: demonstration by enzymatic RNA amplification. J Infect Dis. 1990 Apr;161(4):787–791. doi: 10.1093/infdis/161.4.787. [DOI] [PubMed] [Google Scholar]
  77. Rotbart H. A., Levin M. J., Murphy N. L., Abzug M. J. RNA target loss during solid phase hybridization of body fluids--a quantitative study. Mol Cell Probes. 1987 Dec;1(4):347–358. doi: 10.1016/0890-8508(87)90016-8. [DOI] [PubMed] [Google Scholar]
  78. Rotbart H. A., Levin M. J., Villarreal L. P., Tracy S. M., Semler B. L., Wimmer E. Factors affecting the detection of enteroviruses in cerebrospinal fluid with coxsackievirus B3 and poliovirus 1 cDNA probes. J Clin Microbiol. 1985 Aug;22(2):220–224. doi: 10.1128/jcm.22.2.220-224.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  79. Rotbart H. A., Levin M. J., Villarreal L. P. Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses. J Clin Microbiol. 1984 Dec;20(6):1105–1108. doi: 10.1128/jcm.20.6.1105-1108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  81. Strikas R. A., Anderson L. J., Parker R. A. Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983. J Infect Dis. 1986 Feb;153(2):346–351. doi: 10.1093/infdis/153.2.346. [DOI] [PubMed] [Google Scholar]
  82. Stroop W. G., Baringer J. R., Brahic M. Detection of Theiler's virus RNA in mouse central nervous system by in situ hybridization. Lab Invest. 1981 Dec;45(6):504–509. [PubMed] [Google Scholar]
  83. Stroop W. G., Brahic M., Baringer J. R. Detection of tissue culture-adapted Theiler's virus RNA in spinal cord white matter cells throughout infection. Infect Immun. 1982 Aug;37(2):763–770. doi: 10.1128/iai.37.2.763-770.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  84. Toyoda H., Kohara M., Kataoka Y., Suganuma T., Omata T., Imura N., Nomoto A. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants. J Mol Biol. 1984 Apr 25;174(4):561–585. doi: 10.1016/0022-2836(84)90084-6. [DOI] [PubMed] [Google Scholar]
  85. Tracy S. A comparison of genomic homologies among the coxsackievirus B group: use of fragments of the cloned coxsackievirus B3 genome as probes. J Gen Virol. 1984 Dec;65(Pt 12):2167–2172. doi: 10.1099/0022-1317-65-12-2167. [DOI] [PubMed] [Google Scholar]
  86. Tracy S. Comparison of genomic homologies in the coxsackievirus B group by use of cDNA:RNA dot-blot hybridization. J Clin Microbiol. 1985 Mar;21(3):371–374. doi: 10.1128/jcm.21.3.371-374.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Tracy S., Smith R. A. A comparison of the genomes of polioviruses by cDNA:RNA hybridization. J Gen Virol. 1981 Jul;55(Pt 1):193–199. doi: 10.1099/0022-1317-55-1-193. [DOI] [PubMed] [Google Scholar]
  88. Vuorinen T., Kallajoki M., Hyypiä T., Vainionpä R. Coxsackievirus B3-induced acute pancreatitis: analysis of histopathological and viral parameters in a mouse model. Br J Exp Pathol. 1989 Aug;70(4):395–403. [PMC free article] [PubMed] [Google Scholar]
  89. Wadia N. H., Katrak S. M., Misra V. P., Wadia P. N., Miyamura K., Hashimoto K., Ogino T., Hikiji T., Kono R. Polio-like motor paralysis associated with acute hemorrhagic conjunctivitis in an outbreak in 1981 in Bombay, India: clinical and serologic studies. J Infect Dis. 1983 Apr;147(4):660–668. doi: 10.1093/infdis/147.4.660. [DOI] [PubMed] [Google Scholar]
  90. Wilfert C. M., Lehrman S. N., Katz S. L. Enteroviruses and meningitis. Pediatr Infect Dis. 1983 Jul-Aug;2(4):333–341. doi: 10.1097/00006454-198307000-00019. [DOI] [PubMed] [Google Scholar]
  91. Woods M. G., Diana G. D., Rogge M. C., Otto M. J., Dutko F. J., McKinlay M. A. In vitro and in vivo activities of WIN 54954, a new broad-spectrum antipicornavirus drug. Antimicrob Agents Chemother. 1989 Dec;33(12):2069–2074. doi: 10.1128/aac.33.12.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  92. Yolken R. H., Torsch V. M. Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A. Infect Immun. 1981 Feb;31(2):742–750. doi: 10.1128/iai.31.2.742-750.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  93. Yolken R. H., Torsch V. Enzyme-linked immunosorbent assay for the detection and identification of coxsackie B antigen in tissue cultures and clinical specimens. J Med Virol. 1980;6(1):45–52. doi: 10.1002/jmv.1890060107. [DOI] [PubMed] [Google Scholar]
  94. Yoon J. W., Austin M., Onodera T., Notkins A. L. Isolation of a virus from the pancreas of a child with diabetic ketoacidosis. N Engl J Med. 1979 May 24;300(21):1173–1179. doi: 10.1056/NEJM197905243002102. [DOI] [PubMed] [Google Scholar]
  95. Young N. A. Polioviruses, coxsackieviruses, and echoviruses comparison of the genomes by RNA hybridization. J Virol. 1973 Jun;11(6):832–839. doi: 10.1128/jvi.11.6.832-839.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  96. Yousef G. E., Brown I. N., Mowbray J. F. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology. 1987;28(3):163–170. doi: 10.1159/000150012. [DOI] [PubMed] [Google Scholar]
  97. Zhang H. Y., Yousef G. E., Bowles N. E., Archard L. C., Mann G. F., Mowbray J. F. Detection of enterovirus RNA in experimentally infected mice by molecular hybridisation: specificity of subgenomic probes in quantitative slot blot and in situ hybridisation. J Med Virol. 1988 Dec;26(4):375–386. doi: 10.1002/jmv.1890260405. [DOI] [PubMed] [Google Scholar]
  98. Zurbriggen A., Fujinami R. S. Theiler's virus infection in nude mice: viral RNA in vascular endothelial cells. J Virol. 1988 Oct;62(10):3589–3596. doi: 10.1128/jvi.62.10.3589-3596.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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