Table 3. Results of microinjection with the deletion series of DJ1-TALENs RNA.
Scaffold | RNA concentration (nM) | Injected eggs | Survival at 1 dpf |
---|---|---|---|
N287C230 | Low (9) | 24 | 24 |
High (180) | 24 | 16 | |
N136C230 | Low (9) | 23 | 23 |
High (180) | 24 | 24 | |
N287C63 | Low (9) | 24 | 23 |
High (180) | 24 | 20 | |
N136C63a | Low (9) | 20 | 19 |
High (180) | 20 | 20 | |
N153C47 | Low (9) | 24 | 21 |
High (180) | 24 | 22 | |
N136C47 | Low (9) | 24 | 22 |
High (180) | 22 | 19 |
The results from the N136C63 scaffold are the same as shown for 10 and 200 ng/µl in Table 1.