Figure 2.

OVA-specific T cells in B6.HOD mice are present but do not respond to antigenic challenge. B6 and B6.HOD mice were immunized with OVA/CFA followed by OVA/IFA boost. Anti-OVA IgG were analyzed by (A) flow crossmatch against B6, mHEL, and B6.HOD targets. (B) Total leukocytes from B6 or B6.HOD mice were enumerated by tetramer enrichment assay (C) were analyzed for antigen specificity by comparing tetramer staining (OVA323-339 and OVA326-334 vs. LCMV GP66-77), and (D) were evaluated for activation by anti-CD44 staining. (E) To assess the ability to expand upon challenge, B6 and B6.HOD mice were immunized with OVA323-339 and LCMV61-80 peptides in CFA and subsequently boosted with peptides in IFA. OVA-specific CD4⁺CD44⁺ T cells were enumerated and (F) representative flow plots are provided. (G) LCMV-specific CD4⁺CD44⁺ T cells were also enumerated and (H) activation was assessed by anti-CD44 staining. Representative flow plots are shown. Control CFA-IFA immunizations in the absence of peptides were included as controls. All data are representative of 4 independent experiments with similar results; at least 12 mice were analyzed per group.