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. 1994 Dec;14(12):7884–7890. doi: 10.1128/mcb.14.12.7884

Replication factor A is required in vivo for DNA replication, repair, and recombination.

M P Longhese 1, P Plevani 1, G Lucchini 1
PMCID: PMC359327  PMID: 7969128

Abstract

Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.

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Selected References

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