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DnaG re-primes the leading-strand template downstream of the CPD. Column-isolated CPD-template replication reactions were initiated by EcoRI cleavage in the presence or absence of DnaG for 6 min. Reactions were quenched and digested with either PvuI, or PvuI and EagI, which maps to the region of the template downstream of the CPD (fig. S5B). Samples were analyzed by both native and denaturing gel electrophoresis. The * is as in Fig. 1D.
