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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Transplantation. 2012 Mar 27;93(6):580–588. doi: 10.1097/TP.0b013e318244dd67

Figure 4. Allospecific inhibitory activity of alloantigen induced Tregs.

Figure 4

Alloantigen induced Foxp3+CD25+CD4+ Tregs were differentiated from naïve, polyclonal BALB/c CD4+ T cells using the combination of B6 DCs, IL-2, and TGF-β1. The suppressors tested were (S) were either alloantigen induced Tregs (A) or freshly isolated nTregs (B) and were mixed in various ratios with 105 CD4+CD25− cells from BALB/c [responders(R)], and stimulated with irradiated spleen cells from B6 mice (A and B). (C) AlloTregs generated from BALB/c CD4+ T cells with B6 DCs, IL-2, and TGF-β1 were mixed with 105 CD4+CD25− cells from CBA responder T cells in various ratios, and stimulated with irradiated SJL spleen cells. Proliferation was assessed by [3H]thymidine uptake. Data from each of three consecutive experiments are shown, and expressed as mean (±SE) of three replicates within each experiment. P values calculated using the Kruskal-Wallis test.