Table 2.
Proteins with increased expression in liver of LPS-treated GSNOR −/− mice compared to wild-type control
| Spot no. | t-test | Av. ratio | ID | Protein name | MW (kDa) | P/ | Scorea) | Matchesb) | Coverage (%)c) | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1357 | 0 | 1.73 | ATTY_MOUSE | Tyrosine aminotransferase | 51 | 5.4 | MIS | 82 | 2 | 4 |
| 1471 | 0 | 1.6 | FIBG_MOUSE | Fibrinogen gamma chain | 50 | 5.5 | MIS | 252 | 6 | 17 |
| PMF | 94 | 11 | 28 | |||||||
| RPAB3_MOUSE | DNA-directed RNA polymerases RPABC3 | 17 | 4.5 | PMF | 55 | 6 | 47 | |||
| ARP3_MOUSE | Actin-related protein 3 | 48 | 5.6 | MIS | 47 | 1 | 3 | |||
| 1479 | 0.01 | 2.12 | PLIN2_MOUSE | Perilipin-2 | 47 | 6.4 | MIS | 129 | 3 | 9 |
| PMF | 50 | 6 | 18 | |||||||
| 1683 | 3.20E-10 | 2.46 | ADH4_MOUSE | Alcohol dehydrogenase 4 | 41 | 7.7 | MIS | 83 | 2 | 3 |
| PMF | 57 | 5 | 14 | |||||||
| 1704 | 0.01 | 1.55 | CPSM_MOUSE | Carbamoyl-phosphate synthase | 166 | 6.5 | MIS | 57 | 2 | 1 |
| 1710 | 3.20E-10 | 1.87 | ALDOB_MOUSE | Fructose-bisphosphate aldolase B | 40 | 8.5 | MIS | 157 | 2 | 9 |
| PMF | 69 | 9 | 27 | |||||||
| 1713 | 6.40E-09 | 1.48 | GRP78_MOUSE | 78 kDa glucose-regulated protein | 73 | 5.1 | MIS | 176 | 5 | 9 |
| PMF | 56 | 8 | 15 | |||||||
| 1714 | 0 | 1.42 | ALDOB_MOUSE | Fructose-bisphosphate aldolase B | 40 | 8.5 | MIS | 238 | 5 | 15 |
| PMF | 126 | 13 | 33 | |||||||
| 1785 | 5.60E-05 | 2.58 | ARGI1_MOUSE | Arginase-1 | 35 | 6.5 | PMF | 85 | 8 | 29 |
| 1825 | 0 | 1.68 | FIBG_MOUSE | Fibrinogen gamma chain | 50 | 5.5 | MIS | 32 | 1 | 2 |
| 1963 | 0 | 1.59 | A2M_MOUSE | Alpha-2-macroglobulin | 167 | 6.2 | MIS | 37 | 2 | 0 |
| 2631 | 0 | 2.01 | GPKOW_MOUSE | G patch domain and KOW motifs-containing portein | 54 | 8.2 | PMF | 56 | 6 | 18 |
| 2704 | 2.50E-05 | 1.64 | URAD_MOUSE | 2-Oxo-4-hydroxy-4-carboxy -5-ureidoimidazoline decarboxylase | 20 | 5.7 | MIS | 40 | 1 | 7 |
| PMF | 58 | 5 | 27 | |||||||
| 3644 | 1.30E-09 | 3.57 | S10A9_MOUSE | Protein S100-A9 | 13 | 6.6 | MIS | 129 | 2 | 11 |
| 3659 | 8.90E-05 | 2.72 | S10A9_MOUSE | Protein S100-A9 | 13 | 6.6 | MIS | 154 | 2 | 11 |
| 3679 | 9.60E-05 | 1.51 | CYTB_MOUSE | Cystatin-B | 11 | 6.8 | MIS | 58 | 1 | 9 |
| UK114_MOUSE | Ribonuclease UK114 | 14 | 8.7 | MIS | 42 | 1 | 11 | |||
| 3730 | 1.60E-08 | 7.37 | SAA1_MOUSE | Serum amyloid A-1 protein | 14 | 6.5 | MIS | 25 | 1 | 7 |
| 3732 | 7.70E-06 | 3.19 | SAA1_MOUSE | Serum amyloid A-1 protein | 14 | 6.5 | MIS | 34 | 1 | 7 |
| 3735 | 8.30E-07 | 11.85 | SAA2_MOUSE | Serum amyloid A-2 protein | 14 | 6.4 | MIS | 30 | 1 | 7 |
| 3736 | 3.80E-07 | 4.1 | SAA2_MOUSE | Serum amyloid A-2 protein | 14 | 6.4 | MIS | 37 | 1 | 7 |
| 2930 | 5.10E-06 | 2.94 | APC_MOUSE | Adenomatous polyposis coli protein | 313 | 7.4 | PMF | 58 | 13 | 6 |
a)
Mascot score for proteins identified by MALDI-TOF.
b)
Peptides matched by Mascot search.
c)
Percentage sequence coverage.