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. 1991 Feb;11(2):1107–1113. doi: 10.1128/mcb.11.2.1107

Activation of phosphatidylinositol 3-kinase in cells expressing abl oncogene variants.

L Varticovski 1, G Q Daley 1, P Jackson 1, D Baltimore 1, L C Cantley 1
PMCID: PMC359788  PMID: 1846663

Abstract

A phosphoinositide kinase specific for the D-3 position of the inositol ring, phosphatidylinositol (PI) 3-kinase, associates with activated receptors for platelet-derived growth factor, insulin, and colony-stimulating factor 1, with products of the oncogenes src, fms, yes, crk, and with polyomavirus middle T antigen. Efficient fibroblast transformation by proteins of the abl and src oncogene families requires activation of their protein-tyrosine kinase activity and membrane association via an amino-terminal myristoylation. We have demonstrated that the PI 3-kinase directly associates with autophosphorylated, activated protein-tyrosine kinase variants of the abl protein. In vivo, this association leads to accumulation of the highly phosphorylated products of PI 3-kinase, PI-3,4-bisphosphate and PI-3,4,5-trisphosphate, only in myristoylated, transforming abl protein variants. Myristoylation thus appears to be required to recruit PI 3-kinase activity to the plasma membrane for in vivo activation and correlates with the mitogenicity of the abl protein variants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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