Cell migration and invasion were assessed by counting the number of stained cells that penetrated the migration membrane after 24h following PGE2 (100nM) stimulation. (A) membrane alone (right, stained cells; left migration numbers p<0.05 versus control) (B) matrigel coated membrane (right, stained cells; left migration numbers p<0.05 versus control) (C) RT-PCR showing the expression of HSPG2, COL1A1, elastin, fibronectin, vimentin and (D) the expression of MMP-2, 3, 9, 11 and TIMP-1, 2 before and after stimulation with PGE2 (100nM). β-actin served as control.