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. 1993 Aug;13(8):5020–5033. doi: 10.1128/mcb.13.8.5020

Selective and synchronous activation of early-S-phase replicons of Ehrlich ascites cells.

V Gekeler 1, J Epple 1, G Kleymann 1, H Probst 1
PMCID: PMC360151  PMID: 8336732

Abstract

Twelve-hour exposure of G1 Ehrlich ascites cells to controlled hypoxia (200 ppm of O2 at 1 bar) suppressed replicon initiation. Synchronous cycling, beginning with a normal S phase, was released by reoxygenation immediately. The addition of cycloheximide at reoxygenation largely resuppressed, after a short initial burst, succeeding replicon initiations. Alkaline sedimentation analysis of growing daughter strand DNA, DNA fiber autoradiography, and analysis of the newly formed DNA demonstrated that normal chain growth and DNA maturation (replicon termination) in the initially activated replicons continued in the presence of cycloheximide. After 2 to 3 h, a low level of cycloheximide-insensitive background replication emerged out of the then-ebbing single surge of activity of the initially released replicons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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